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Triazine mediated covalent antibiotic grafting on cotton fabrics as a modular approach for developing antimicrobial barriers

  • Ana Maria Montagut
  • Albert Granados
  • Caitlin Lazurko
  • Antony El-Khoury
  • Erik J. Suuronen
  • Emilio I. AlarconEmail author
  • Rosa María SebastiánEmail author
  • Adelina VallriberaEmail author
Original Research


New antimicrobial textiles were prepared through direct chemical linkage of bioactive molecules eugenol and fluoroquinolone derivatives, onto the surface of cotton fabrics. The attachment through a triazine moiety minimizes the leaching of the antimicrobial molecule into the surroundings of the material. Bacterial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa was studied. The treated textile with fluoroquinolone demonstrated bacteriostatic antimicrobial effects having a tendency to decrease the population of S. aureus in the planktonic form. A significant effect was also observed in the prevention of S. aureus biofilm formation and in its ability to kill bacteria within a preformed biofilm. Eugenol-modified fabric was also active in the process of eradicating preformed P. aeruginosa biofilms. Further, in vitro assays using human dermal fibroblast cells indicate no effects on cell proliferation and viability, and in vivo tests in a murine skin wound model showed no increase of IL-6 for full-thickness wounds that were in contact with the fabrics.

Graphic abstract


Microbicidal Cotton fabrics Covalent functionalization Fluoroquinolone Biocompatibility Biofilm destruction 



Financial support for this work was provided by the Spanish Ministerio de Ciencia, Innovación y Universidades (Grants CTQ2014-53662-P, RTI2018-097853-B-I00 and 2016-81797-REDC) and by Generalitat de Catalunya (2017 SGR 00465). EIA and EJS thanks to the Canadian Institutes of Health Research (CIHR) for financial support. EIA also thanks the support of NSERC through the Discovery Grant program. AEK is appreciative to University of Ottawa for an Undergraduate Research Opportunity Award. CL is thankful for the Queen Elizabeth II Graduate Scholarship in Science and Technology.

Supplementary material

10570_2019_2584_MOESM1_ESM.doc (54.1 mb)
Supplementary material 1 (DOC 55356 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ana Maria Montagut
    • 1
  • Albert Granados
    • 1
  • Caitlin Lazurko
    • 2
    • 3
  • Antony El-Khoury
    • 2
  • Erik J. Suuronen
    • 2
  • Emilio I. Alarcon
    • 2
    • 3
    Email author
  • Rosa María Sebastián
    • 1
    Email author
  • Adelina Vallribera
    • 1
    Email author
  1. 1.Department of Chemistry and Centro de Innovación en Química (ORFEO-CINQA)Universitat Autònoma de BarcelonaCerdanyola del VallèsSpain
  2. 2.Division of Cardiac SurgeryUniversity of Ottawa Heart InstituteOttawaCanada
  3. 3.Department of Biochemistry, Microbiology, and Immunology, Faculty of MedicineUniversity of OttawaOttawaCanada

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