Improved thermostability and cytocompatibility of bacterial cellulose/collagen composite by collagen fibrillogenesis
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Composite bacterial cellulose (BC) membranes containing collagen fibrils or collagen monomers (named BC/Col-fibre or BC/Col, respectively) were fabricated by immersing macroporous BC in a 3.0 mg/mL collagen solution for 50 h at 4 °C with or without collagen fibrillogenesis before lyophilization. The structure of the membranes was characterized by scanning electron microscope, nitrogen adsorption–desorption experiment, and X-ray diffraction. The structure became much more compact upon the introduction of collagen but the crystal structure of BC did not change. The thermal stability and the cytocompatibility of the membranes were evaluated by thermogravimetric analysis and a cell adhesion assay, respectively. The thermal stability of BC was enhanced by collagen incorporation and by fibrillogenesis. The adhesive ability and the proliferation of NIH/3 T3 fibroblast cells in BC/Col-fibre were better than that in BC/Col, which was still superior to that in BC.
KeywordsCollagen Bacterial cellulose Fibrillogenesis Thermostability Cytocompatibility
This study was financially supported by the National Natural Science Foundation of China (Nos. 21676208, 21706201, 21376183), the Wuhan Morning Light Plan of Youth Science and Technology (No. 2017050304010326), the Hubei Provincial Natural Science Foundation of China (Nos. 2018CFA030, 2017CFB507), and the Innovation Team Program of Hubei province, China (No. T201208).
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Conflict of interest
All authors declare that they have no conflict of interest.
- Zhang Q, Hubenak J, Iyyanki T, Alred E, Turza KC, Davis G, Chang EI, Branch-Brooks CD, Beahm EK, Butler CE (2015) Engineering vascularized soft tissue flaps in an animal model using human adipose–derived stem cells and VEGF + PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle. Biomaterials 73:198–213CrossRefGoogle Scholar