Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide
- 33 Downloads
In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%.
KeywordsDressings Acemannan grafting Cotton Response surface methodology Biocompatibility Antibacterial
The authors would like to acknowledge the University of Monastir, and, Tunisian Ministry of Higher Education and Scientific Research, for giving the opportunity and making easy, the achievement of this work. In addition, the authors thank the Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products in the Faculty of Pharmacy, for biological studies. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Compliance with ethical standards
Conflict of interest
The authors declared that they have no conflict of interest.
- Box GE, Wilson KB (1951) On the experimental attainment of optimum conditions. J R Stat Soc: Ser B (Methodological) 13:1–38Google Scholar
- Chandegara V, Varshney A (2013) Aloe vera L. processing and products: a review. Int J Med Aromat Plants 3:492–506Google Scholar
- El Ghoul Y, Salah F, Majdoub H, Sakli F (2016) Synthesis and study of drug delivery system obtained via β-cyclodextrin functionalization of viscose/polyester dressings. J Ind Text 1528083716652833Google Scholar
- Lumbreras-Aguayo A, Meléndez-Ortiz HI, Puente-Urbina B, Alvarado-Canché C, Ledezma A, Romero-Garcia J, Betancourt-Galindo R (2019) Poly (methacrylic acid)-modified medical cotton gauzes with antimicrobial and drug delivery properties for their use as wound dressings. Carbohydr Polym 205:203–210CrossRefGoogle Scholar
- Salah F, El Ghoul Y, Roudesli S (2016) Bacteriological effects of functionalized cotton dressings. J Text Inst 107:171–181Google Scholar