, Volume 26, Issue 5, pp 3219–3230 | Cite as

The influence of lignin content and structure on hemicellulose alkaline extraction for non-wood and hardwood lignocellulosic biomass

  • Wenhui Geng
  • Robert Narron
  • Xiao Jiang
  • Joel J. Pawlak
  • Hou-min Chang
  • Sunkyu Park
  • Hasan Jameel
  • Richard A. VendittiEmail author
Original Research


The extractability of hemicellulose from different lignocellulosics depends on the source of biomass. Differences in hemicellulose extractability are believed to be due to plant-specific hemicellulose arrangement alongside lignin within the cell wall. In this research, six biomasses were used to probe hemicellulose alkaline extractability as a function of the native lignin within the biomasses. Quantitative 2D-HSQC and 13C NMR analysis were performed to determine the S/G (S: syringyl, G: guaiacyl) and lignin-carbohydrate complex (LCC) linkages of milled wood lignin isolated from these biomasses. A strong negative correlation was observed between total lignin content and hemicellulose extractability, demonstrating that a greater presence of lignin in the original material results in lower xylan solubilization. In addition, a correlation between S/G of lignin and xylan dissolution was found within a group of hardwoods and within a group of non-woods. This suggests that monomeric constituency also influences xylan’s propensity for dissolution in 10% NaOH. Although there is some uncertainty in the quantification of LCC linkages, both non-woods and hardwoods exhibited negative correlations between alkaline-stable LCC linkages content and xylan extractability. This suggests that alkaline-stable LCC structures are associated with a decrease in the alkaline extractability of hemicellulose.

Graphical abstract


Hemicellulose Alkaline extraction Lignin S/G Lignin-carbohydrate complex 



This research was supported by the Southeastern Sun Grant Regional Program of the USDA-NIFA program [Grant Number 2013-38502-21423].

Supplementary material

10570_2019_2261_MOESM1_ESM.docx (878 kb)
Supplementary material 1 (DOCX 878 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Wenhui Geng
    • 1
  • Robert Narron
    • 1
  • Xiao Jiang
    • 1
  • Joel J. Pawlak
    • 1
  • Hou-min Chang
    • 1
  • Sunkyu Park
    • 1
  • Hasan Jameel
    • 1
  • Richard A. Venditti
    • 1
    Email author
  1. 1.Department of Forest BiomaterialsNorth Carolina State UniversityRaleighUSA

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