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Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/cellulose nanocrystal films: artificial weathering, humidity absorption, water vapor transmission rate, antimicrobial activity and biocompatibility

  • Sara Malmir
  • Luis Barral
  • Rebeca BouzaEmail author
  • Marta Esperanza
  • Marta Seoane
  • Sandra Feijoo-Bandín
  • Francisca Lago
Original Research
  • 40 Downloads

Abstract

Bionanocomposite films of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with different cellulose nanocrystal (CNC) loadings were prepared. Effects of artificial weathering, humidity absorption, water vapor transmission rate (WVTR), antimicrobial activity and biocompatibility were studied. Differential scanning calorimetry (DSC) and time resolved synchrotron X-ray diffraction were used to provide information about the effect of CNC on the crystalline structure of PHBV in the bionanocomposites. The incorporation of CNC accelerated the crystallization rate of PHBV due to a nucleating effect. DSC results showed first a decrease of crystallization and then the occurrence of secondary crystallization after artificial weathering. Scanning electron microscopy images implied well CNC distribution before artificial weathering and formation of blister—like points due to artificial weathering. Hydrophobicity of bionanocomposites was found to be slightly lower than for the PHBV in the humidity absorption tests. Bionanocomposites exhibited smaller WVTR due to restricted PHBV chain mobility. Samples with zinc oxide and silver showed antimicrobial inhibition activities against S. enterica, L. monocytogenes, S. aureus and E. coli. Results of cell viability and nitrite accumulation tests showed that PHBV and its bionanocomposites were biocompatible.

Graphical abstract

Keywords

PHBV CNC Artificial weathering Biocompatibility Time resolved synchrotron X-ray diffraction Thermal analysis Antimicrobial activity 

Notes

Acknowledgments

This work was supported by the Ministerio de Economía y Competitividad [MAT2013-41892-R, Project NanoCompBioPol] and the Xunta de Galicia Government/FEDER: Program of Consolidation and Structuring Competitive Research Units [GRC 2014/036]. The synchrotron experiments were performed at beam line BL11—NCD at ALBA synchrotron Light Facility with the collaboration of ALBA staff. R. Bouza acknowledges the grant of ALBA synchrotron installation.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Universitaria PolitécnicaUniversidade da CoruñaFerrolSpain
  2. 2.Grupo MICROALGAE, Departamento de Biología, Facultad de CienciasUniversidade da CoruñaA CoruñaSpain
  3. 3.Cellular and Molecular Cardiology Research Unit, Institute of Biomedical Research (IDIS-SERGAS)University Clinical HospitalSantiago de CompostelaSpain
  4. 4.Center for Biomedical Research Network in Cardiovascular Diseases (CIBERCV)MadridSpain

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