Nanocellulose biosynthesis by Komagataeibacter hansenii in a defined minimal culture medium
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This study evaluates the capability of Komagataeibacter hansenii ATCC 23769 to synthesize bacterial nanocellulose (BNC) under strict limitation of nutrients with different carbon and nitrogen sources in a defined minimal culture medium in static culture. Five carbon sources were prepared based on the C-molar basis concentration: glycerol, glucose, fructose, mannitol and saccharose, combined with three different nitrogen sources: NH4Cl, NH4NO3 and (NH4)2SO4. BNC production yields were determined based on the dry weight of the produced BNC-Minimal membranes and the carbon consumption for each condition. The combination of 25 mM of glucose and 10 mM of NH4Cl showed the best concentration of C and N sources regarding BNC yield and membrane stability. This is a new proposal of defined minimal culture medium that supports growth of BNC membranes production, without the addition of complex elements such as yeast extract and peptone. BNC membranes produced under these conditions exhibited great optical transparency compared to the membranes produced in complex media (Mannitol and Hestrin–Schramm), therefore increasing a wide range of technological applications.
KeywordsKomagataeibacter hansenii Bacterial nanocellulose Defined minimal culture medium Carbon and nitrogen sources Transparency
The authors thank the National Council for the Improvement of Higher Education (CAPES), for the financial support. The authors also thank the Central Laboratory of Electronic Microscopy at Federal University of Santa Catarina (LCME/UFSC) and the Analytical Center of the Chemical and Food Engineering Department (EQA/UFSC).
Funding was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Grant No. 1407463/2014-1) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant No. 402901/2013-4).
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Conflict of interest
All authors have agreed to submit the manuscript to the Cellulose. The authors declare no conflict of interest.
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