, Volume 25, Issue 12, pp 7113–7125 | Cite as

Hybrid scaffolds enhanced by nanofibers improve in vitro cell behavior for tissue regeneration

  • Baoxiu Wang
  • Chengsheng Huang
  • Shiyan Chen
  • Xueyu Xing
  • Minghao Zhang
  • Qingkai Wu
  • Huaping Wang
Original Paper


To construct a biomimetic scaffold with a nanoscale structure similar to that of natural tissue, the objective of this work was to prepare three-dimensional (3D) porous hybrid scaffolds based on gelatin and bacterial cellulose nanofibers for tissue regeneration. The nanofibrous structure, water absorption, and compressive mechanical properties of the scaffolds were studied. The hybrid scaffolds not only give a sufficiently porous structure for efficient nutrient transport and vascularization, but also provide the nanofibrous structure and improve the roughness of the scaffold pore walls. The hybrid scaffolds also exhibit higher modulus as stiffness compared to the pure gelatin scaffold. The viability and morphology of Pig iliac endothelial cells (PIECs) cultured on the 3D scaffolds were examined. PIECs adhered and proliferated better on the stiff hybrid scaffold with nanofibers compared to the soft gelatin scaffold without nanofibers. The results addressed the effect of the nanofibers and the stiffness of scaffolds on cell behavior, and the biomimetic nanofibrous hybrid scaffolds would be highly favorable/desired for tissue regeneration, e.g., skin and urethral regeneration.

Graphical Abstract


Nanofibers Porous 3D scaffold Stiffness Tissue regeneration 



This work was supported by the National Natural Science Foundation of China (51573024, 81771523 and 81550008), the Fundamental Research Funds for the Central Universities (17D310612), and DHU Distinguished Young Professor Program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10570_2018_2087_MOESM1_ESM.doc (916 kb)
Supplementary material 1 (DOC 916 kb)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and EngineeringDonghua UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Obstetrics and Gynecology, Affiliated Sixth People’s HospitalShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

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