Advertisement

Cellulose

pp 1–16 | Cite as

Isolation of xylans from bleached Eucalyptus kraft pulp by antisolvents precipitation

  • Ana Isabel MarquesEmail author
  • Maria de Lurdes Serrano
  • Ana Maria Brites Alves
  • António P. Mendes de Sousa
Original Paper
First Online:
  • 114 Downloads

Abstract

The present work is focused on alkaline extraction of xylans from bleached Kraft pulp of Eucalyptus globulus, and its isolation from the alkaline extract, aiming high purity product and yields. Alkaline extraction was optimized, through the stirring speed and extraction time. A higher stirring speed increased extraction yields which varied between 65 and 85%. However, extraction time did not have influence on extraction yields. Xylans isolation was performed by precipitation with diluted acids and with various alcohol concentrations. Washing of the precipitates, to remove impurities, was performed either with water and/or with methanol. Water washing yielded xylans with purity values up to 99%, while with methanol those values were lower, in the range of 36–84%. Xylans characterization indicated average molecular weights of 16.8–22.7 kDa. The isolated xylan yields varied between 43 and 97%. Nitric acid showed to be the most adequate solvent to get pure xylan. On the other hand, in alcohol experiments lower yields were obtained due to the losses in the subsequent water washing process.

Keywords

Eucalyptus globulus Bleached Kraft pulp Alkaline extraction Precipitation Xylans 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

The authors wish to thank to Prof. Ana Paula Dias (IST) for her help in FTIR and thermogravimetric analysis, to RAIZ for the collaboration in HPAEC analysis and pentosan determination and to Prof. Dmitry Evtuguin and Dr. Ana Reis from Aveiro University for the collaboration in GPC analysis.

References

  1. Albalasmeh AA, Berhe AA, Ghezzehei TA (2013) A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohyd Polym 97(2):253–261Google Scholar
  2. Barbosa B, Colodette J, Muguet M, Gomes V, Oliveira R (2016). Effects of xylan in Eucalyptus pulp production. In: CERNE, vol 22(2). Universidade Federal de Lavras abr./jun. 2016Google Scholar
  3. Cai CM, Zhang T, Kumar R, Wyman CE (2014) Integrated furfural production as a renewable fuel and chemical platform from lignocellulosic biomass. J Chem Technol Biotechnol 89:2–10Google Scholar
  4. Cantu-Jungles TM, Iacomini M, Cipriani TR, Cordeiro LMC (2017) Isolation and characterization of a xylan with industrial and biomedical applications from edible açaí berries (Euterpe oleraceae). Food Chem 221:1595–1597Google Scholar
  5. Colodette JL, Longue D Jr, Pedrazzi C, Oliveira RC, Gomide JL, Gomes FJB (2011) Pulpability and bleachability of xylan-depleted eucalyptus wood chips. Ind Eng Chem Res 50:1847–1852Google Scholar
  6. Da Magaton AS, Piló-Veloso D, Colodette J (2008) Caracterização das O-acetil-(4-O-metilglucorono) xilanas isoladas da madeira de Eucalyptus Urograndis. Quim Nova 31(5):1085–1088Google Scholar
  7. Deutschmann R, Dekker RFH (2012) From plant biomass to bio-based chemicals: latest developments in xylan research. Biotechnol Adv 30:1627–1640Google Scholar
  8. Ebringerova A, Heinze T (2000) Xylan and xylan derivatives—biopolymers with valuable properties, 1—naturally occurring xylans structures, procedures and properties. Macromol Rapid Commun 21(9):542–556Google Scholar
  9. Ebringerova A, Hromadkova Z (2010) An overview on the application of ultrasound in extraction, separation and purification of plant polysaccharides. Cent Eur J Chem 8:243–257Google Scholar
  10. Eduardo da Silva A, Marcelino HR, Gomes MCS, Oliveira EE, Nagashima Jr T, Egito EST (2012) Xylan, a promising hemicellulose for pharmaceutical use. In: Verbeek J (ed) Products and applications of biopolymers. InTech, pp 61–84Google Scholar
  11. Evtuguin DV, Neto CP (2007) Recent advances in Eucalyptus wood chemistry: structural features through the prism of technological response. https://www.researchgate.net/publication/237632161
  12. Evtuguin DV, Neto CP, Tomás JL, Silva AMS (2003) Characterization of an acetylated heteroxylan from Eucalyptus globulus Labill. Carbohyd Res 338:597–604Google Scholar
  13. Gabrielii I, Gatenholm P, Glasser WG, Jain RK, Kenne L (2000) Separation, characterization and hydrogel-formation of hemicellulose from aspen wood. Carbohyd Polym 43:367–374Google Scholar
  14. Hakala TK, Liitiä T, Suurnäkki A (2013) Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood Kraft pulp. Carbohyd Polym 93:102–108Google Scholar
  15. Hettrich K, Drechsler U, Loth F, Volkert B (2017) Preparation and characterization of water soluble xylan ethers. Polymers 9(4):129Google Scholar
  16. Laine C, Kemppainen K, Kuuti L, Varhimo A, Asikainen S, Maattanen M, Buchert J, Harli A (2015) Extraction of xylan from wood pulp and brewer’s spent grain. Ind Crops Prod 70:231–237Google Scholar
  17. Li X, Shi X, Wang M, Du Y (2011) Xylan chitosan conjugate—a potential food preservative. Food Chem 126(2):520–525Google Scholar
  18. Lisboa SA, Evtuguin DV, Neto CP, Goodfellow BJ (2005) Isolation and structural characterization of polysaccharides dissolved in Eucalyptus globulus Kraft black liquors. Carbohyd Polym 60:77–85Google Scholar
  19. Luo Q, Peng H, Zhou M, Lin D, Ruan R, Wan Y, Jinsheng Z, Yuhuan L (2012) Alkali extraction and physicochemical characterization of hemicelluloses from young Bamboo (Phyllostachys Pubescens Mazel). Bioresources 7(4):5817–5828Google Scholar
  20. Monrroy M, Mendonça RT, Ruiz J, Baeza J, Freer J (2009) Estimating glucan, xylan, and methylglucoronic acids in kraft pulps of Eucalyptus globulus using FT-NIR spectroscopy and multivariate analysis. J Wood Chem Technol 29(2):150–163Google Scholar
  21. Monrroy M, García J-R, Mendonça R, Baeza J, Freer J (2012) Kraft pulping of Eucalyptus globulus as a pretreatment for bioethanol production by simultaneous saccharification and fermentation. J Chil Chem Soc 57(2):1113–1117Google Scholar
  22. Mullin JW (2001) Crystallization, 4th edn. Butterworth Heinemann, Oxford, pp 480–491Google Scholar
  23. Naidu D, Hlangothi S, John M (2018) Bio-based products from xylan: a review. Carbohyd Polym 179:28–41Google Scholar
  24. Palmeiras L, Magaton A, Colodette J, Carvalho A (2010) Análise comparativa entre vários métodos de quantificação de hemiceluloses da madeira de eucalipto. Quim Nova 33(7):1569–1571Google Scholar
  25. Peng F, Ren J-L, Xu F, Bian J, Peng P, Sun RC (2009) Comparative study of hemicelluloses obtained by graded ethanol precipitation from sugarcane bagasse. J Agric Food Chem 57:6305–6317Google Scholar
  26. Peng F, Bian J, Peng P, Sun RC, Ren Jun-Li, Xu Feng (2010) Fractionation of alkali-solubilized hemicelluloses from delignified Populus gansuensis: structure and properties. J Agric Food Chem 58(9):5743–5750Google Scholar
  27. Peng F, Bian J, Jia N, Peng P, Sun RC, Liu SJ (2012) Isolation and fractionation of hemicelluloses from Salix Psammophila. Cellul Chem Technol 46(3–4):177–184Google Scholar
  28. Perry RH, Chilton CH (1973) Chemical engineering handbook, 5th edn. McGraw-Hill, New York, pp 3–94 (Solubilities) Google Scholar
  29. Pinto PC, Evtuguin DV, Neto CP (2005) Structure of hardwood glucuronoxylans: modifications and impact on pulp retention during wood Kraft pulping. Carbohyd Polym 60:489–497Google Scholar
  30. Ramos A, Sousa S, Evtuguin D, Gamelas J (2017) Functionalized xylans in the production of xylan-coated paper laminates. React Funct Polym 117:89–96Google Scholar
  31. Romaní A, Ruiz HA, Pereira FB, Domingues L, Teixeira JA (2014) Effect of hemicellulose liquid phase on the enzymatic hydrolysis of autohydrolyzed Eucalyptus globulus wood. Biomass Convers Biorefinery 4(2):77–86Google Scholar
  32. Saake B, Kruse T, Puls J (2001) Investigation on molar mass, solubility and enzymatic fragmentation of xylans by multi-detected SEC chromatography. Biores Technol 80:195–204Google Scholar
  33. Sárossy Z (2011) Production and utilization of hemicelluloses from renewable resources for sustainable advanced products. Doctoral dissertation, Technical University of Denmark—Biosystems DivisionGoogle Scholar
  34. Shatalov AA, Pereira H (2002) Carbohydrate behavior of Arundodonax L. in ethanol-alkali medium of variable composition during organosolv delignification. Carbohyd Polym 49:331–336Google Scholar
  35. Silva SS, Carvalho RR, Fonseca JLC, Garcia RB (1998) Extracção e Caracterização de Xilanas de Sabugos de Milho. Polímeros: Ciência e Tecnologia 8(2):25–33Google Scholar
  36. Silva FF, Alves AB, Serrano ML, Sousa AM (2017) Isolation and purification of concentrated and non-concentrated hemicellulose alkaline extracts. Sep Purif Technol 173:233–239Google Scholar
  37. Singh RD, Banerjee J, Sasmal S, Muir J, Arora A (2018) High xylan recovery using two stage alkali pre-treatment process from high lignin biomass and its valorisation to xylooligosaccharides of low degree of polymerization. Biores Technol 256:110–117Google Scholar
  38. Spork D, Reinoso FAM, Rencoret J, Gutiérrez A, del Rio JC, Ferraz A, Milagres AMF (2017) Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. Biotechnol Biofuels 10:1–11Google Scholar
  39. Tappi T223 cm-84 (1984) Pentosans in wood and pulpGoogle Scholar
  40. Teramoto N, Mottoyama T, Yosomiya R, Shibata M (2002) Eur Polym J 39:255–261Google Scholar
  41. Ünlu CH, Günister E, Atici O (2009) Synthesis and characterization of NaMt biocomposites with corn cob xylan in aqueous media. Carbohydr Polym 76(4):585–592Google Scholar
  42. Valls C, Pastor FIJ, Vidal T, Roncero MB, Díaz P, Martínez J, Valenzuela SV (2018) Antioxidant activity of xylooligosaccharides produced from glucuronoxylan by Xyn10A and Xyn30D xylanases and Eucalyptus autohydrolysates. Carbohyd Polym 194(15):43–50Google Scholar
  43. Werner K, Pommer L, Broström M (2014) Thermal decomposition ofhemicelluloses. J Anal Appl Pyrol 110:130–137Google Scholar
  44. Xue B-L, Wn J-L, Xu F, Sun R-C (2012) Structural characterization of hemiceluloses fractionated by graded ethanol precipitation from Pinus yunnanensis. Carbohyd Res 352:159–165Google Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.CERENA, Chemical Engineering DepartmentInstituto Superior TécnicoLisbonPortugal
  2. 2.CeFEMA, Chemical Engineering DepartmentInstituto Superior TécnicoLisbonPortugal
  3. 3.RAIZ, Instituto de Investigação da Floresta e PapelEixoPortugal

Personalised recommendations

Cite article

Buy options