Analysis of galactoglucomannans from spruce wood by capillary electrophoresis
- 202 Downloads
The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.
KeywordsCapillary electrophoresis Carbohydrates Galactoglucomannan Glucomannan Polysaccharide
4-Amino benzoic acid ethyl ester
Capillary zone lectrophoresis
Sodium dodecyl sulphate
This work is the part of EPNOE (European Polysaccharide Network of Excellence) activity.
- Hannuksela T, Holmbom B (2004) Sorbtion of mannans to different fiber surfaces: an evolution of understanding, American Chemical SocietyGoogle Scholar
- Heinze T (2005) Polysaccharides I, structure, characterization and use, advances in polymer science. Springer, BerlinGoogle Scholar
- Lagane B, Treilhou M, Couderc F (2000) Capillary electrophoresis: theory, teaching approach and separation of oligosaccharides using indirect UV detection. Biochem Mol Biol Educ 28(5):251–255Google Scholar
- Ristolainen M, Alén R (2001) Carbohydrates from chemical pulps: characterization by capillary zone electrophoresis. Encyclopedia of Analytical Chemistry: Applications, Theory, and Instrumentation, pp 8345–8361Google Scholar
- Sjöström E (1993) Wood chemistry-fundamentals and applications, 2nd edn. Academic Press, New YorkGoogle Scholar
- Woodward C, Weinberger R (1994) Capillary zone electrophoresis of carbohydrates derivatized with 4-aminobenzoic acid ethyl ester. Aplication Note, Hewlett PackardGoogle Scholar