Study on the Deacidification of Rice Bran Oil Esterification by Magnetic Immobilized Lipase

  • Dianyu Yu
  • Changhua Yu
  • Tong Wang
  • Jun Chen
  • Xin Zhang
  • Liqing Wang
  • Lanxia QinEmail author
  • Fei Wu


Candida antarctica lipase B (CALB) was immobilized on Fe3O4/SiOx-g-P (GMA) polymer carrier to obtain nano-magnetic Fe3O4/SiOx-g-P (GMA) lipase (magnetic enzyme). The obtained magnetic enzyme particles had an enzyme protein loading of 98.7 mg/g, an enzyme activity of 1226.5 U/g, an average particle diameter of 100.5 ± 1.30 nm, a magnetization of 15.80 emu/g, a magnetic enzyme optimum pH of 7.0, and an optimum temperature of 50 °C. The prepared magnetic enzyme was used as a catalyst, phytosterol was esterified with free fatty acid (FFA) in rice bran oil (RBO). Under the conditions of pH was 7.0, the FFA/phytosterol molar ratio was 1:4, the dosage of the immobilized CALB was 10.0%, the esterification temperature was 50 °C, the esterification time was 72 h, and the molecular sieve addition was 8.0%. The FFA content in RBO decreased from 16.0 to 2.4%, and the conversion rate of phytosterol was 85.7%. When the magnetic enzyme was used repeatedly for 7 times, the relative enzyme activity of the magnetic enzyme was 83.0%, which improved the utilization efficiency of the enzyme.

Graphic Abstract


Nano-magnetic Fe3O4/SiOx-g-P (GMA) lipase Rice bran oil Esterification deacidification FFA Phytosterol 



This work was also supported by a grant from Rice bran high-value steady-state processing technology and intelligent equipment development and demonstration (No.: 2018YFD0401101). Additionally, this work was supported by a grant from the Province Natural Science Foundation of Heilong Jang: Study on the Mechanism of Continuous Orientation Esterification of Nanometer Magnetic Lipase (No.: C2017019). This work was supported by a grant from the National Natural Science Foundation of China (NSFC): Study on the mechanism of nanomagnetic enzyme hydrolysis of soybean oil by multi-effect orientation and biosynthesis of functional lipids (No.: 31571880). The authors would like to thank the anonymous reviewers and the editor for their comments on an earlier version of this paper.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dianyu Yu
    • 1
  • Changhua Yu
    • 1
  • Tong Wang
    • 1
  • Jun Chen
    • 1
  • Xin Zhang
    • 1
  • Liqing Wang
    • 2
  • Lanxia Qin
    • 1
    Email author
  • Fei Wu
    • 1
  1. 1.School of Food ScienceNortheast Agricultural UniversityHarbinChina
  2. 2.School of Computer and Information EngineeringHarbin University of CommerceHarbinChina

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