Facile Fabrication of a Novel and Reusable 3D Laccase Reactor for Efficient Removal of Pollutants from Wastewater

  • Yulin Zou
  • Fanpeng Ran
  • Qiangwei Huang
  • Xiaoyan Liu
  • Haixia ZhangEmail author



An ultra simple 3D bioreactor was prepared via Fe3+ linking to primary amines of melamine sponge (MeS) for cooperative complex formation with enzyme. Laccase worked as a model of enzyme and no chemical modification was employed during the material preparation. The 3D structure of MeS can enhance the enzyme loading amount up to 180 µg/mg, albeit MeS as monolithic material for enzyme immobilization is rather unexplored. The 3D bioreactor (MeS@Fe3+@laccase) also improved the catalytic efficiency and showed excellent reusability, stability, and reproducibility. In this study, the as-prepared bioreactor (10 mg) was successfully applied to the degradation of dyes and micropollutants, which has shown higher efficiency than free laccase in the large-scale decolorization of dyes and showed up to almost 100% removal of 2,4,6-trichlorophenol (2,4,6-TCP) and sulfisoxazole. Therefore, the MeS@Fe3+ with low cost, good stability and easy separation can be employed as a new enzyme carrier such as trypsin and lipase in biotechnological applications.

Graphical Abstract


Enzyme immobilization Laccase Melamine sponge Dye Sulfonamides 2,4,6-TCP 



This work was financially supported by the National Natural Science Foundation of China (21575055).

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

Supplementary material

10562_2019_2732_MOESM1_ESM.doc (9.8 mb)
Supplementary material 1 (DOC 10017 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhouChina

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