Evaluating the adhesion of human gingival fibroblasts and MG-63 osteoblast-like cells to activated PRP-coated membranes

  • Surena Vahabi
  • Zahra Yadegary
  • Mohamadjavad KaramshahiEmail author


Regeneration of periodontal tissues is affected by the biological and morphological characteristics of the membrane surface. The current study evaluated the adhesion of human gingival fibroblasts (HGF) and MG-63 osteoblast-like cells to Membranes, with and without activated PRP. The line of human gingival fibroblast cells and MG-63 osteoblast-like cells were first prepared and cultured on three types of membranes, including Jason, CenoMembrane and TXT-200 in three groups (FBS 10%, FBS 0.5% and activated PRP). Cell viability was investigated by MTT assay and electron microscopy (SEM) was used to evaluate the cell morphology and adhesion on these membranes after 24 and 72 h. Two-way ANOVA was carried out at the significance level of 0.05. The highest adhesion in the 10% FBS group for HGF and The MG-63 osteoblast-like cells was observed to the Jason membrane during 24 h and 72 h (p < 0.05). However, there were no significant differences among the three membranes in PRP and FBS groups for HGF during 24 h and for MG-63 cells during 72 h (p > 0.05). Activated PRP had a positive effect on the viability and adhesion of both human gingival fibroblasts and osteoblast-like cells as compared to the FBS 0.5% group, but these effects were not as 10% FBS group. The results also showed that Jason membrane had the highest amount of cell viability and adhesion.


Cell adhesion Guided tissue regeneration Fibroblasts Osteoblasts Platelet-rich plasma 



This article is based on the Thesis No. 822 written by Dr Karamshahi under supervision of Dr Vahabi and related to the School of Dentistry, Shahid Beheshti University of Medical Sciences. The authors would like to express their gratitude to the Cellular Molecular Oral Biology Laboratory of School of Dentistry, Shahid Beheshti University of Medical Sciences for providing technical support for this study.


This study was funded by Shahid Beheshti University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

Platelet-rich plasma was obtained from volunteers, after explaining the objective of the study and providing a summary of the research stages as well as obtaining their informed verbal and written consent. Gingival fibroblasts and osteoblast-like cells were also prepared from Cell Bank Department of Pasteur institute. Also, the methodology of this experimental study was consistent to all the ethical protocols approved by the University’s Ethics Committee and registered with the code of IR.SBMU.RIDS.REC.1396.440 at the Ethics Committee.

Informed consent

Informed verbal and written consent was obtained from volunteers for extracting platelet-rich plasma. Also, the methodology of this experimental study was consistent to all the ethical protocols approved by the University’s Ethics Committee and registered with the code of IR.SBMU.RIDS.REC.1396.440 at the Ethics Committee.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Albandar JM (2002) Periodontal diseases in North America. Periodontol 2000(29):3–69. Google Scholar
  2. Albandar JM, Brunelle JA, Kingman A (1999) Destructive periodontal disease in adults 30 years of age and older in the United States, 1988–1994. J Periodontol 70:13–29. CrossRefGoogle Scholar
  3. Altiere ET, Reeve CM, Sheridan PJ (1979) Lyophilized bone allografts in periodontal intraosseous defects. J Periodontol 50:510–519. CrossRefGoogle Scholar
  4. Arora NS, Ramanayake T, Ren YF, Romanos GE (2010) Platelet-rich plasma in sinus augmentation procedures: a systematic literature review: part II. Implant Dent 19:145–157. CrossRefGoogle Scholar
  5. Barekatain M, Mafi M, Amini S, Farhad SZ (2014) Emdogain effect on gingival fibroblast adhesion in bioabsorbable and non-resorbable barrier membranes: an in vitro study. Dent Res J 11:429–435Google Scholar
  6. Boyapati L, Wang HL (2006) The role of platelet-rich plasma in sinus augmentation: a critical review. Implant Dent 15:160–170. CrossRefGoogle Scholar
  7. Caceres M, Hidalgo R, Sanz A, Martínez J, Riera P, Smith PC (2008) Effect of platelet-rich plasma on cell adhesion, cell migration, and myofibroblastic differentiation in human gingival fibroblasts. J Periodontol 79:714–720. CrossRefGoogle Scholar
  8. Camargo PM, Lekovic V, Weinlaender M, Vasilic N, Madzarevic M, Kenney EB (2005) A reentry study on the use of bovine porous bone mineral, GTR, and platelet-rich plasma in the regenerative treatment of intrabony defects in humans. Int J Periodontics Restorative Dent 25:49–59Google Scholar
  9. Caton J, Nyman S, Zander H (1980) Histometric evaluation of periodontal surgery II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol 7:224–231. CrossRefGoogle Scholar
  10. Celotti F, Colciago A, Negri-Cesi P, Pravettoni A, Zaninetti R, Sacchi MC (2006) Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts: role of platelet-derived growth factor and transforming growth factor-β. Wound Repair Regen 14:195–202. CrossRefGoogle Scholar
  11. Chang T, Liu Q, Marino V, Bartold PM (2007) Attachment of periodontal fibroblasts to barrier membranes coated with platelet-rich plasma. Aust Dent J 52:227–233. CrossRefGoogle Scholar
  12. Creeper F, Lichanska AM, Marshall RI, Seymour GJ, Ivanovski S (2009) The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation. J Periodontal Res 44:258–265. CrossRefGoogle Scholar
  13. Del Fabbro M, Bortolin M, Taschieri S (2011) Is autologous platelet concentrate beneficial for post-extraction socket healing? A systematic review. Int J Oral Maxillofac Surg 40:891–900. CrossRefGoogle Scholar
  14. Del Fabbro M, Bortolin M, Taschieri S, Weinstein RL (2013) Effect of autologous growth factors in maxillary sinus augmentation: a systematic review. Clin Implant Dent Relat Res 15:205–216. CrossRefGoogle Scholar
  15. Ghasemi M, Foroutan T, Nikzad M (2013) The study of non-absorbable membrane compatibility to human fibroblast periodontal ligament. Pajoohandeh J 18:128–132Google Scholar
  16. Gonzalez AC, Costa TF, Andrade ZA, Medrado AR (2016) Wound healing—A literature review. An Bras Dermatol 91:614–620. CrossRefGoogle Scholar
  17. Hämmerle CH, Jung RE, Feloutzis A (2002) A systematic review of the survival of implants in bone sites augmented with barrier membranes (guided bone regeneration) in partially edentulous patients. J Clin Periodontol 29:226–231CrossRefGoogle Scholar
  18. Kasaj A, Reichert C, Götz H, Röhrig B, Smeets R, Willershausen B (2008) In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration. Head Face Med 4:22. CrossRefGoogle Scholar
  19. Lang NP, Lindhe J (eds) (2015) Clinical Periodontology and Implant Dentistry, vol 2. Wiley, HobokenGoogle Scholar
  20. Lee SW, Kim SG (2014) Membranes for the guided bone regeneration. Maxillofac Plast Reconstr Surg 36:239–246. CrossRefGoogle Scholar
  21. Moraschini V, Barboza EDSP (2016) Use of platelet-rich fibrin membrane in the treatment of gingival recession: a systematic review and meta-analysis. J Periodontol 87:281–290. CrossRefGoogle Scholar
  22. Nguyen PA, Pham TAV (2018) Effects of platelet-rich plasma on human gingival fibroblast proliferation and migration in vitro. J Appl Oral Sci 26:e20180077. CrossRefGoogle Scholar
  23. Nieminen T, Kallela I, Keränen J, Hiidenheimo I, Kainulainen H, Wuolijoki E, Rantala I (2006) In vivo and in vitro degradation of a novel bioactive guided tissue regeneration membrane. Int J Oral Maxillofac Surg 35:727–732. CrossRefGoogle Scholar
  24. Nyman S, Gottlow J, Karring T, Lindhe J (1982) The regenerative potential of the periodontal ligament. J Clin Periodontol 9:257–265. CrossRefGoogle Scholar
  25. Oryan A, Alidadi S, Moshiri A (2016) Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther 16:213–232. CrossRefGoogle Scholar
  26. Paolantonio M, Perinetti G, Dolci M, Perfetti G, Tetè S, Sammartino G, Femminella B, Graziani F (2008) Surgical treatment of periodontal intrabony defects with calcium sulfate implant and barrier versus collagen barrier or open flap debridement alone: a 12-month randomized controlled clinical trial. J Periodontol 79:1886–1893. CrossRefGoogle Scholar
  27. Pihlstrom BL, McHuon RB, Oliphant TH, Ortiz-Campos C (1983) Comparison of surgical and nonsurgical treatment of periodontal disease.A review of current studies and additional results after 6 1/2 years. J Clin Periodontol 10:524–541. CrossRefGoogle Scholar
  28. Rattanasuwan K, Rassameemasmaung S, Kiattavorncharoen S, Sirikulsathean A, Thorsuwan J, Wongsankakorn W (2018) Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells. Eur J Dent 12:469–474. CrossRefGoogle Scholar
  29. Rothamel D, Schwarz F, Sculean A, Herten M, Scherbaum W, Becker J (2004) Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Clin Oral Implant Res 15:443–449. CrossRefGoogle Scholar
  30. Shah M, Deshpande N, Bharwani A, Nadig P, Doshi V, Dave D (2014) Effectiveness of autologous platelet-rich fibrin in the treatment of intra-bony defects: a systematic review and meta-analysis. J Indian Soc Periodontol 18:698–704. CrossRefGoogle Scholar
  31. Sharma A, Pradeep AR (2011) Autologous platelet-rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. J Periodontol 82:1396–1403. CrossRefGoogle Scholar
  32. Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FQ (2007) Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease. J Dent Res 86:306–319. CrossRefGoogle Scholar
  33. Takata T, Wang HL, Miyauchi M (2001) Attachment, proliferation and differentiation of periodontal ligament cells on various guided tissue regeneration membranes. J Periodontal Res 36:322–327. CrossRefGoogle Scholar
  34. Terranova VP, Martin GR (1982) Molecular factors determining gingival tissue interaction with tooth structure. J Periodontal Res 17:530–533. CrossRefGoogle Scholar
  35. Vahabi S, Yadegari Z, Mohammad-Rahimi H (2017) Comparison of the effect of activated or non-activated PRP in various concentrations on osteoblast and fibroblast cell line proliferation. Cell Tissue Bank 18:347–353. CrossRefGoogle Scholar
  36. Wang HL, Miyauchi M, Takata T (2002) Initial attachment of osteoblasts to various guided bone regeneration membranes: an in vitro study. J Periodontal Res 37:340–344. CrossRefGoogle Scholar
  37. Xu Y, Jiang Y, Lin X, Bartold PM (2007) Human osteoblasts attachment to guided tissue regeneration membranes which were coated either with platelet-rich plasma or platelet-poor plasma. Zhonghua Kou Qiang Yi Xue Za Zhi 42:496–500Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Periodontics, School of DentistryShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Department of Dental Biomaterials, School of DentistryShahid Beheshti University of Medical SciencesTehranIran

Personalised recommendations