Effect of antibiotic impregnation time on the release of gentamicin from cryopreserved allograft bone chips: an in vitro study
- 4 Downloads
Freezing is the most common method for storing bones until use in skeletal reconstruction. However, the effect of freezing at different temperatures on antibiotic delivery from antibiotic-coated bone chips has not been evaluated. In this study, we compared antibiotic delivery in vitro from gentamicin-coated human bone stored at different temperatures impregnated for different time periods. Bone chips obtained from human femur heads were chemically cleaned and mixed with gentamicin sulfate solution for 1 h and 10 h respectively. Samples of both groups were cryopreserved for 4 months at − 20 °C, 4 months at − 80 °C, or evaluated immediately without freezing. Antibiotic release from the bone chips was measured using Bacillus subtilis as an indicator strain. Zones of inhibition and rates of gentamicin were higher for the samples impregnated for 10 h as compared to 1 h. There was no significant difference between non-cryopreservation, cryopreservation at different temperatures of − 20 and − 80 °C on the release of gentamicin from bone chips even after storage for 4 months.
KeywordsBone transplantation Allograft Gentamicin Storage Bone bank
The authors gratefully thank Dr. Archana Thakur, Director, G.B. Pant Hospital, New Delhi for her support and contribution to this study. We would also like to thank the Department of Microbiology, G.B. Pant Hospital, New Delhi for their help with the completion of this study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Informed Consent was obtained from the tissue donor.
- Anagnostakos K, Schroder K (2012) Antibiotic-impregnated bone grafts in orthopaedic and trauma surgery: a systematic review of the literature. Int J Biomater 2012, Article ID 538061, 9pGoogle Scholar
- Atkinson BA (1986) Species incidence and trends of susceptibility to antibiotics in the United States and other countries: MIC and MBC. In: Antibiotics in laboratory medicine, 2nd edn, Williams & Wilkins, Baltimore, pp 1086–1089Google Scholar
- Chambers WB (1977) Pallagrosi AU 1977 Gentamicin in the treatment of staphylococcal infections. J Int Med Res 5(6):442–449Google Scholar
- Friedlander GE (1987a) Bone banking in support of reconstructive surgery of the hip. Clin Orthop Relat Res. Dec, (225):17–21Google Scholar
- Friedlander GE (1987b) Bone Grafts- the basic science rationale of clinical application. J Bone Joint Surg Am. Jun; 69(5):786–90Google Scholar