Cell and Tissue Banking

, Volume 20, Issue 1, pp 77–86 | Cite as

Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis

  • F. Mohammadi
  • N. Varanloo
  • M. Heydari Nasrabadi
  • A. Vatannejad
  • F. S. Amjadi
  • M. Javedani Masroor
  • L. Bajelan
  • M. Mehdizadeh
  • R. Aflatoonian
  • Z. ZandiehEmail author


The aim of this study is to evaluate the beneficial effect of Myoinositol (MYO) supplement in freezing media on the post thaw sperm quality. Semen samples from 40 normozoospermic men were divided into two aliquots and frozen with simple or 2 mg/mL MYO supplemented freezing medium. Post thaw process including, computer-assissted sperm analysis was used to analyze sperm motility and morphology. Reactive oxygen species was evaluated by the fluorometry of DCFH-DA, as well as total antioxidant capacity and lipid peroxidation were measured based on colorimetric assay by ELISA reader. Eventually, DNA fragmentation was assessed using TUNEL staining. MYO significantly improved progressive motility and normal morphology in treated samples (p < 0.05). Lipid peroxidation (malondialdehyde level) can be diminished in samples were frozen by MYO supplemented freezing media (p < 0.05). While MYO did not affect the amount of ROS (p > 0.05), it was associated with high values of total antioxidant capacity (p < 0.05). DNA integrity was significantly affected by MYO, as in MYO treated samples, DNA fragmentation was decreased compared to control ones (p < 0.001). The findings support the use of 2 mg/mL myoinositol supplemented freezing media in sperm cryopreservation to increase sperm quality after freezing–thawing procedures.


Myoinositol Human sperm cryopreservation Sperm parameters Apoptosis DNA fragmentation 



We wish to thank Dr.Joghataei for his kind courtesy and attempt to improve the article.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the national research committee.

Financial support

This research was supported by Shahid Akbarabadi Clinical Research Unit, Iran University Of Medical Sciences.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • F. Mohammadi
    • 1
    • 2
  • N. Varanloo
    • 3
  • M. Heydari Nasrabadi
    • 4
  • A. Vatannejad
    • 5
    • 6
  • F. S. Amjadi
    • 7
    • 2
  • M. Javedani Masroor
    • 2
  • L. Bajelan
    • 8
  • M. Mehdizadeh
    • 7
  • R. Aflatoonian
    • 9
  • Z. Zandieh
    • 2
    • 7
    Email author
  1. 1.Anatomy Department, School of MedicineIran University of Medical ScienceTehranIran
  2. 2.Shahid Akbar Abadi Clinical Research Development Unit (ShACRDU)Iran University of Medical Sciences (IUMS)TehranIran
  3. 3.Department of Basic Sciences, Central Tehran BranchIslamic Azad UniversityTehranIran
  4. 4.Department of Biology Sciences, Parand BranchIslamic Azad UniversityTehranIran
  5. 5.Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and DieteticsTehran University of Medical SciencesTehranIran
  6. 6.Students’ Scientific Research Center (SSRC)Tehran University of Medical SciencesTehranIran
  7. 7.School of Medicine, Cellular and Molecular Research CenterIran University of Medical SciencesTehranIran
  8. 8.Mehr IVF DepartmentMehr HospitalTehranIran
  9. 9.Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive BiomedicineACECRTehranIran

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