Cell and Tissue Banking

, Volume 20, Issue 1, pp 35–48 | Cite as

Deep frozen amniotic membrane used as a scaffold and/or carrier for different cell types

  • Jana Dragúňová
  • Peter KabátEmail author
  • Valéria Cucorová
  • Marianna Hajská
  • Ján Koller


Amniotic membrane is a biological material widely used in plastic and reconstructive surgery and in ophthalmology. Due to its excellent biocompatibility and strength we tried to use it as a scaffold for the in vitro cultivation of different cell types, especially keratinocytes and limbal stem cells. It was possible to cultivate limbal stem cells and keratinocytes without using 3T3 mouse fibroblast feeder cells on deep frozen amniotic membranes. The amniotic membrane can also be used as a carrier for suspensions of different types of cells, allowing a substantial reduction of the cultivation time needed to prepare cell cultures for clinical application to burn patients. Our results show that the amniotic membrane seems not only to be an excellent carrier for human keratinocytes and corneal limbal stem cells, but also for other cell types, including dermal fibroblasts, adipose tissue-derived mesenchymal stem cells and chondrocytes.


Amniotic membrane Keratinocytes Limbal stem cells Dermal fibroblasts Mesenchymal stem cells Chondrocytes 



Amniotic membrane




Central Tissue Bank


Limbal stem cells


Mesenchymal stem cells


Human dermal fibroblasts

R-G method

Cultivation by Rheinwald and Green





This contribution is the result of the project implementation “Development of the Centre of Excellence for Exploitation of Informational Biomacromolecules for Improvement of Quality of Life” supported by the Research and Development Operational Programe funded by the ERDF (Contract No. ITMS 26240120027) and project “Irradiated Scaffolds for Tissue Engineering of Skin” supported by IAEA (International Atomic energy Agency) Contract Nr: 18282, CRP Code: E 31007.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflicts of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Burns and Reconstructive Surgery, Faculty of MedicineComenius UniversityBratislavaSlovakia
  2. 2.Department of Microbiology and Virology, Faculty of Natural SciencesComenius UniversityBratislava 4Slovakia
  3. 3.Institute of Virology, Biomedical Research Center SASBratislavaSlovakia
  4. 4.Department of Burns and Reconstructive SurgeryUniversity HospitalBratislavaSlovakia

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