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Cellular cardiomyoplasty: development of a technique to culture human myoblasts for clinical transplantation

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Abstract

Some recent studies have demonstrated that epicardial injection of autologous myoblasts, obtained from satellite cells of skeletal muscle, in association to coronary artery bypass graft surgery (CABG) in patients with decreased left ventricular function secondary to ischaemic disease could be of some utility to get a better recovery of ventricular function due to the ability of these cells to grow and generate new muscle fibers over the previous fibrotic scar. The aims are the setting up of a process for the collection of the cellular cardiomyoplasty in samples of multiorganic donations and to carry out this technique in the same surgical moment as the revascularisation is performed in two patients. For this purpose we obtained muscle through biopsy of 15 human multiorgan donors and of two patients. Separation of fatty tissue, minced, and further digestion with collagenase type I (1.5 mgr/ml/2 gr by weight) and trypsin 1 ×. Filtration of the cellular suspension, centrifugation and sowing of this suspension in culture medium, with 20% of human serum. Culture for three weeks until obtainment of between 200–300 million cells. Inmunohistochemistry and flow cytometry for the identification of the myoblasts was carried out. The results were obtained through flow cytometry, using CD56 as an indicator of the presence of myoblasts, between 70 and 80% of these types of cells were obtained after three weeks of culture. By inmunohistochemistry analyses, different markers were analyzed: desmin and myogenin. The results indicated the presence of a great number of positive cells with these markers, possibly myoblasts. Skeletal myoblast implant was not associated with adverse effects. The culture of autologous myoblasts is a rapid and simple technique where after three weeks of culture a great number of cells for implantation are obtained. In patients with old myocardial infarction, treatment with skeletal myoblast in conjunction with coronary artery bypass is safe and feasible. and it is easy to obtain myoblasts from muscle tissue for transplant into patients.

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Authors and Affiliations

  1. Cryobiology Unit, Complejo Hospitalario Juan Canalejo, La Coruña, Spain

    M. Esther Rendal Vázquez, M. Rodriguez Cabarcos & C. Andión Núñez

  2. Unit of Investigation, Complejo Hospitalario Juan Canalejo, La Coruña, Spain

    T. Diaz Román, N. Doménech Garcia, M.J. Sánchez Dopico & F.J. Blanco Garcia

  3. Department of Cardiac Surgery, Complejo Hospitalario Juan Canalejo, La Coruña, Spain

    A. Juffé Stein & B. Adrio Nazar

  4. Unit of Cryobiology, Carretera del Pasaje s/n Hospital Teresa Herrera, 15006, La Coruña

    M. Esther Rendal Vázquez

Authors
  1. M. Esther Rendal Vázquez
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  2. M. Rodriguez Cabarcos
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  3. T. Diaz Román
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  4. A. Juffé Stein
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  5. N. Doménech Garcia
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  6. B. Adrio Nazar
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  7. M.J. Sánchez Dopico
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  8. C. Andión Núñez
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  9. F.J. Blanco Garcia
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Correspondence to M. Esther Rendal Vázquez.

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Vázquez, M.E.R., Cabarcos, M., Román, T. et al. Cellular cardiomyoplasty: development of a technique to culture human myoblasts for clinical transplantation. Cell Tissue Banking 6, 117–124 (2005). https://doi.org/10.1007/s10561-004-7990-4

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  • DOI: https://doi.org/10.1007/s10561-004-7990-4

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