Temporal reliability of serum soluble and endogenous secretory receptors for advanced glycation end-products (sRAGE and esRAGE) in healthy women
The soluble receptor for advanced glycation end-products (sRAGE) and endogenous secretory RAGE (esRAGE) have been considered as biomarkers of several chronic diseases. However, the temporal reliability of their concentrations in the circulation is yet to be demonstrated. We evaluated whether a single measurement of serum sRAGE and esRAGE could serve as an estimate for usual serum levels in epidemiologic studies.
Serum sRAGE and esRAGE were measured using ELISAs in three yearly samples from 36 participants in the New York University Women’s Health Study. The intraclass correlation coefficient (ICC) was used to evaluate temporal reliability.
The intra- and inter-batch coefficients of variation were 3.0% and 14.8% for sRAGE and 6.5% and 34.7% for esRAGE, and decreased to 0.4% and 2.1% for sRAGE and 1.0% and 6.3% for esRAGE after log2-transformation of the data. On the original scale, the ICCs of a single measurement of serum sRAGE and esRAGE were 0.89 (95% CI 0.82–0.94) and 0.87 (95% CI 0.79–0.93), respectively, and were similar using log2-transformed data.
Our results indicate that a single measurement of serum sRAGE and esRAGE is a sufficiently reliable measure of their usual levels that can be used in epidemiologic studies.
KeywordssRAGE esRAGE Biomarker Reliability ELISA Epidemiology
This work was supported by the National Institutes of Health (Grant Numbers UM1CA182934, P30 CA016087, and P30 ES000260).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
The study was approved by the Institutional Review Board of New York University School of Medicine.
Written informed consent was obtained from all the subjects at the time of enrolment.
- 3.Yonekura H, Yamamoto Y, Sakurai S et al (2003) Novel splice variants of the receptor for advanced glycation end-products expressed in human vascular endothelial cells and pericytes, and their putative roles in diabetes-induced vascular injury. Biochem J 370:1097–1109CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Koenig KL, Toniolo P, Bruning PF, Bonfrer JM, Shore RE, Pasternack BS (1993) Reliability of serum prolactin measurements in women. Cancer Epidemiol Biomark Prevent 2:411–414Google Scholar
- 22.Colhoun HM, Betteridge DJ, Durrington P et al (2011) Total soluble and endogenous secretory receptor for advanced glycation end products as predictive biomarkers of coronary heart disease risk in patients with type 2 diabetes: an analysis from the CARDS trial. Diabetes 60:2379–2385CrossRefPubMedPubMedCentralGoogle Scholar