Advertisement

Biomedical Microdevices

, 21:14 | Cite as

Monitoring drug pharmacokinetics and immunologic biomarkers in dermal interstitial fluid using a microneedle patch

  • Chandana Kolluru
  • Mikayla Williams
  • Jihee Stephanie Yeh
  • Richard K. Noel
  • Jennifer Knaack
  • Mark R. PrausnitzEmail author
Article
  • 58 Downloads

Abstract

Minimally invasive point-of-care diagnostic devices are of great interest for rapid detection of biomarkers in diverse settings. Although blood is the most common source of biomarkers, interstitial fluid (ISF) is an alternate body fluid that does not clot or contain red blood cells that often complicate analysis. However, ISF is difficult to collect. In this study, we assessed the utility of a microneedle patch to sample microliter volumes of ISF in a simple and minimally invasive manner. We demonstrated the use of ISF collected in this way for therapeutic drug monitoring by showing similar vancomycin pharmacokinetic profiles in ISF and serum from rats. We also measured polio-specific neutralizing antibodies and anti-polio IgG in ISF similar to serum in rats immunized with polio vaccine. These studies demonstrate the potential utility of ISF collected by microneedle patch in therapeutic drug monitoring and immunodiagnostic applications.

Keywords

Interstitial fluid Microneedle patch Biomarker Vancomycin Polio specific antibodies Therapeutic drug monitoring 

Notes

Acknowledgments

The authors would like to thank William Weldon of the CDC for conducting serum and ISF neutralizing antibody assays and Donna Bondy for administrative support. Mark Prausnitz is an inventor of patents that have been or may be licensed to companies developing microneedle-based products, is a paid advisor to companies developing microneedle-based products and is a founder/shareholder of companies developing microneedle-based products, including Micron Biomedical. These potential conflicts of interest have been disclosed and are being managed by Georgia Tech and/or Emory University.

References

  1. N.L. Anderson, N.G. Anderson, Mol. Cell. Proteomics 1(11), 845 (2002)CrossRefGoogle Scholar
  2. E. Caffarel-Salvador, A.J. Brady, E. Eltayib, T. Meng, A. Alonso-Vicente, P. Gonzalez-Vazquez, B.M. Torrisi, E.M. Vicente-Perez, K. Mooney, D.S. Jones, PLoS One 10(12), e0145644 (2015)CrossRefGoogle Scholar
  3. J.E. Celis, P. Gromov, T. Cabezón, J.M. Moreira, N. Ambartsumian, K. Sandelin, F. Rank, I. Gromova, Mol. Cell. Proteomics 3(4), 327 (2004)CrossRefGoogle Scholar
  4. H. Chang, M. Zheng, X. Yu, A. Than, R.Z. Seeni, R. Kang, J. Tian, D.P. Khanh, L. Liu, P. Chen. Adv. Mater. 29(37) (2017)Google Scholar
  5. J.W. Coffey, S.C. Meliga, S.R. Corrie, M.A. Kendall, Biomaterials 84, 130 (2016)CrossRefGoogle Scholar
  6. B. Deacon, J. Abramowitz, J. Anxiety Disord. 20(7), 946 (2006)CrossRefGoogle Scholar
  7. R.F. Donnelly, K. Mooney, E. Caffarel-Salvador, B.M. Torrisi, E. Eltayib, J.C. McElnay, Ther. Drug Monit. 36(1), 10 (2014)Google Scholar
  8. G.J. Downing, Biomarkers: Facing the Challenges at the Crossroads of Research and Health Care, Pharmaceutical Science Encyclopedia, John Wiley & Sons, Inc., pp. 1 (2010)Google Scholar
  9. C. Edens, N.C. Dybdahl-Sissoko, W.C. Weldon, M.S. Oberste, M.R. Prausnitz, Vaccine 33(37), 4683 (2015)CrossRefGoogle Scholar
  10. A. El-Laboudi, N.S. Oliver, A. Cass, D. Johnston, Diabetes Technol. Ther. 15(1), 101 (2013)CrossRefGoogle Scholar
  11. D.J. Finney, Statistical Method in Biological Assay, Charles Griffin: London (1952)Google Scholar
  12. F.T. Fischbach, M.B. Dunning, A manual of laboratory and diagnostic tests, Lippincott Williams & Wilkins (2009)Google Scholar
  13. N. Fogh-Andersen, B.M. Altura, B.T. Altura, O. Siggaard-Andersen, Clin. Chem. 41(10), 1522 (1995)Google Scholar
  14. S.K. Garg, R.O. Potts, N.R. Ackerman, S.J. Fermi, J.A. Tamada, H.P. Chase, Diabetes Care 22(10), 1708 (1999)CrossRefGoogle Scholar
  15. S.A. Geherin, S.R. Fintushel, M.H. Lee, R.P. Wilson, R.T. Patel, C. Alt, A.J. Young, J.B. Hay, G.F. Debes, J. Immunol. 1102639 (2012)Google Scholar
  16. B. Greenwood, A. Hall, M. Rowe, H. Whittle, M. George, A. Al-Ghassani, M. Elbualy, P. Malankar, A. Suleiman, G. Clements, Bull. World Health Organ. 74, 253 (1996)Google Scholar
  17. J. Hadrévi, B. Ghafouri, A. Sjörs, H. Antti, B. Larsson, A. Crenshaw, B. Gerdle, F. Hellström, Eur. J. Appl. Physiol. 113(12), 2977 (2013)CrossRefGoogle Scholar
  18. Y. Hamada, J.L. Kuti, D.P. Nicolau, J. Antimicrob. Chemother. 70(7), 2064 (2015)CrossRefGoogle Scholar
  19. C. Herkenne, I. Alberti, A. Naik, Y.N. Kalia, F.-X. Mathy, V. Préat, R.H. Guy, Pharm. Res. 25(1), 87 (2008)CrossRefGoogle Scholar
  20. S.T. Housman, A.A. Bhalodi, A. Shepard, J. Nugent, D.P. Nicolau, J. Am. Podiatr. Med. Assoc. 105(5), 381 (2015)CrossRefGoogle Scholar
  21. Y. Ito, Y. Inagaki, S. Kobuchi, K. Takada, T. Sakaeda, Int. J. Med. Sci. 13(4), 271 (2016)CrossRefGoogle Scholar
  22. T.K. Kiang, V. Schmitt, M.H. Ensom, B. Chua, U.O. Häfeli, J. Pharm. Sci. 101(12), 4642 (2012)CrossRefGoogle Scholar
  23. T.K. Kiang, U.O. Häfeli, M.H. Ensom, Clin. Pharmacokinet. 53(8), 695 (2014)CrossRefGoogle Scholar
  24. U. Kiistala, K. Mustakallio, J. Investig. Dermatol. 48(5), 466 (1967)CrossRefGoogle Scholar
  25. M. Lindberger, T. Tomson, L. Ståhle, Basic Clin. Pharmacol. Toxicol. 91(4), 158 (2002)CrossRefGoogle Scholar
  26. D. Loewenstein, C. Stake, M. Cichon, Am. J. Emerg. Med. 31(8), 1236 (2013)CrossRefGoogle Scholar
  27. D. Mabey, R.W. Peeling, A. Ustianowski, M.D. Perkins, Nat. Rev. Microbiol. 2(3), 231 (2004)CrossRefGoogle Scholar
  28. P.R. Miller, S.D. Gittard, T.L. Edwards, D.M. Lopez, X. Xiao, D.R. Wheeler, N.A. Monteiro-Riviere, S.M. Brozik, R. Polsky, R.J. Narayan, Biomicrofluidics 5(1), 013415 (2011)CrossRefGoogle Scholar
  29. P.R. Miller, S.A. Skoog, T.L. Edwards, D.M. Lopez, D.R. Wheeler, D.C. Arango, X. Xiao, S.M. Brozik, J. Wang, R. Polsky, Talanta 88, 739 (2012)CrossRefGoogle Scholar
  30. P.R. Miller, X. Xiao, I. Brener, D.B. Burckel, R. Narayan, R. Polsky, Adv. Healthcare Mater. 3(6), 876 (2014)CrossRefGoogle Scholar
  31. A.C. Müller, F.P. Breitwieser, H. Fischer, C. Schuster, O. Brandt, J. Colinge, G. Superti-Furga, G. Stingl, A. Elbe-Bürger, K.L. Bennett, J. Proteome Res. 11(7), 3715 (2012)CrossRefGoogle Scholar
  32. D.A. Muller, S.R. Corrie, J. Coffey, P.R. Young, M.A. Kendall, Anal. Chem. 84(7), 3262 (2012)CrossRefGoogle Scholar
  33. J. Nachbaur, M. Clarke, J. Provost, J. Dancla, Lab. Anim. Sci. 27(6), 972 (1977)Google Scholar
  34. M.M. Niedzwiecki, P. Samant, D.I. Walker, V. Tran, D.P. Jones, M.R. Prausnitz, G.W. Miller, Anal. Chem. 90(6), 3786 (2018)CrossRefGoogle Scholar
  35. S. Pichini, I. Altieri, P. Zuccaro, R. Pacifici, Clin. Pharmacokinet. 30(3), 211 (1996)CrossRefGoogle Scholar
  36. S.A. Plotkin, W.A. Orenstein, P.A. Offit, Vaccines, Saunders (2004)Google Scholar
  37. M.R. Prausnitz, Annu. Rev. Chem. Biomol. Eng. 8, 177 (2017)CrossRefGoogle Scholar
  38. E. Renard, Curr. Diabetes Rev. 4(3), 169 (2008)CrossRefGoogle Scholar
  39. M.J. Rybak, Clin. Infect. Dis. 42(Supplement_1), S35 (2006)CrossRefGoogle Scholar
  40. M. Rybak, B. Lomaestro, J.C. Rotschafer, R. Moellering, W. Craig, M. Billeter, J.R. Dalovisio, D.P. Levine, Am. J. Health Syst. Pharm. 66(1), 82 (2009)CrossRefGoogle Scholar
  41. P.P. Samant, M.R. Prausnitz, Proc. Natl. Acad. Sci. 115, 4583 (2018)Google Scholar
  42. S. Schmidt, R. Banks, V. Kumar, K.H. Rand, H. Derendorf, J. Clin. Pharmacol. 48(3), 351 (2008)CrossRefGoogle Scholar
  43. A. Sieg, R.H. Guy, M.B. Delgado-Charro, Clin. Chem. 50(8), 1383 (2004)CrossRefGoogle Scholar
  44. C.H. Sloop, L. Dory, P.S. Roheim, J. Lipid Res. 28(3), 225 (1987)Google Scholar
  45. P. Stetina, B. Madai, V. Kulemann, W. Kirch, C. Joukhadar, Int. J. Clin. Pharmacol. Ther. 43(3), 134 (2005)CrossRefGoogle Scholar
  46. H. Suh, J. Shin, Y.-C. Kim, Clin. Exp. Vaccine Res. 3(1), 42 (2014)CrossRefGoogle Scholar
  47. B.Q. Tran, P.R. Miller, R.M. Taylor, G. Boyd, P.M. Mach, C.N. Rosenzweig, J.T. Baca, R. Polsky, T. Glaros, J. Proteome Res. 17(1), 479 (2017)CrossRefGoogle Scholar
  48. M. Venugopal, K.E. Feuvrel, D. Mongin, S. Bambot, M. Faupel, A. Panangadan, A. Talukder, R. Pidva, IEEE Sensors J. 8(1), 71 (2008)CrossRefGoogle Scholar
  49. A. Vrdoljak, E.A. Allen, F. Ferrara, N.J. Temperton, A.M. Crean, A.C. Moore, J. Control. Release 225, 192 (2016)CrossRefGoogle Scholar
  50. H. Wiig, O. Tenstad, P.O. Iversen, R. Kalluri, R. Bjerkvig, Fibrog. Tissue Repair 3(1), 12 (2010)CrossRefGoogle Scholar
  51. P. Yager, G.J. Domingo, J. Gerdes, Annu. Rev. Biomed. Eng. 10, 107 (2008)CrossRefGoogle Scholar
  52. Y. Ye, J. Yu, D. Wen, A.R. Kahkoska, Z. Gu, Adv. Drug Deliv. Rev. 127, 106 (2018)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chandana Kolluru
    • 1
  • Mikayla Williams
    • 1
  • Jihee Stephanie Yeh
    • 2
  • Richard K. Noel
    • 3
  • Jennifer Knaack
    • 2
  • Mark R. Prausnitz
    • 1
    Email author
  1. 1.School of Chemical and Biomolecular EngineeringGeorgia Institute of TechnologyAtlantaUSA
  2. 2.School of Pharmaceutical SciencesMercer UniversityAtlantaUSA
  3. 3.Physiological Research LaboratoryGeorgia Institute of TechnologyAtlantaUSA

Personalised recommendations