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LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)

  • Liang Wan
  • Jie Gao
  • Tianlan Chen
  • Cheng Dong
  • Haoran Li
  • Yan-Zi Wen
  • Zhao-Rong Lun
  • Yanwei JiaEmail author
  • Pui-In Mak
  • Rui P. Martins
Article

Abstract

A major goal in the development of point-of-care (POC) devices is to build them as portable to provide a rapid and effective determination for disease pathogens. In nucleic acid testing, an optical detection system used to monitor the product of nucleic acid amplification has always been a bulky accessory. In this work, we developed a handheld, automatic and detection system-free thermal digital microfluidic (DMF) device for DNA detection by loop-mediated isothermal amplification (LAMP). Droplet manipulation and real-time temperature control systems were integrated into a handheld device. The control software could be installed into any tablet and communicate with the device via Bluetooth. In the experimentation, we loaded 2-μl samples with an electrowetting force into sandwich-structured DMF chips, thereby considerably reducing reagent consumptions. After an on-chip LAMP reaction, we added a highly concentrated SYBR Green I droplet and mixed it with a reaction droplet to enable product detection with the naked eye. This step prevented aerosol contamination by avoiding the exposure of the reaction droplet to the air. Using a blood parasite Trypanosoma brucei as a model system, this system showed similar results as a commercial thermal cycler and could detect 40 copies per reaction of the DNA target. This low-cost, compact device removed the bulky optical system for DNA detection, thus enabling it to be well suited for POC testing.

Keywords

Digital microfluidic system Handheld device Loop-mediated isothermal amplification (LAMP) Naked-eye visualisation 

Notes

Acknowledgements

This work was supported by FDCT110/2016/A3 and AMSV SKL Fund from the Macao Science and Technology Development Fund (FDCT), MYRG2017-00022-AMSV and SRG2016-00072-AMSV from the University of Macau, and #31720103918 from the National Natural Science Foundation of China.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Analog and Mixed-Signal VLSIUniversity of MacauMacao SARChina
  2. 2.Department of ECE, Faculty of Science and TechnologyUniversity of MacauMacao SARChina
  3. 3.Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life SciencesSun Yat-Sen UniversityGuangzhouChina
  4. 4.On leave from Instituto Superior TécnicoUniversidade de LisboaLisboaPortugal

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