Biomedical Microdevices

, 20:66 | Cite as

Multiplexed microarrays based on optically encoded microbeads

  • Atieh Vafajoo
  • Azin Rostami
  • Sanam Foroutan Parsa
  • Reza Salarian
  • Navid Rabiee
  • Ghazal Rabiee
  • Mohammad Rabiee
  • Mohammadreza TahririEmail author
  • Daryoosh Vashaee
  • Lobat Tayebi
  • Michael R. HamblinEmail author


In recent years, there has been growing interest in optically-encoded or tagged functionalized microbeads as a solid support platform to capture proteins or nucleotides which may serve as biomarkers of various diseases. Multiplexing technologies (suspension array or planar array) based on optically encoded microspheres have made possible the observation of relatively minor changes in biomarkers related to specific diseases. The ability to identify these changes at an early stage may allow the diagnosis of serious diseases (e.g. cancer) at a time-point when curative treatment may still be possible. As the overall accuracy of current diagnostic methods for some diseases is often disappointing, multiplexed assays based on optically encoded microbeads could play an important role to detect biomarkers of diseases in a non-invasive and accurate manner. However, detection systems based on functionalized encoded microbeads are still an emerging technology, and more research needs to be done in the future. This review paper is a preliminary attempt to summarize the state-of-the-art concerning diagnostic microbeads; including microsphere composition, synthesis, encoding technology, detection systems, and applications.


Microbeads Optical encoding Multiplexing technologies Early diagnosis 



The work was supported by the Faculty of Biomedical Engineering, Amirkabir University of Technology. Michael R Hamblin was supported by US National Institute of Health (NIH) under Grants R01AI050875 and R21AI121700.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Atieh Vafajoo
    • 1
  • Azin Rostami
    • 1
  • Sanam Foroutan Parsa
    • 1
  • Reza Salarian
    • 2
  • Navid Rabiee
    • 3
  • Ghazal Rabiee
    • 3
  • Mohammad Rabiee
    • 1
  • Mohammadreza Tahriri
    • 4
    Email author
  • Daryoosh Vashaee
    • 5
  • Lobat Tayebi
    • 4
    • 6
  • Michael R. Hamblin
    • 7
    • 8
    • 9
    Email author return OK on get
  1. 1.Biomaterials Group, Faculty of Biomedical EngineeringAmirkabir University of TechnologyTehranIran
  2. 2.Biomedical Engineering DepartmentMaziar UniversityNoorIran
  3. 3.Department of ChemistryShahid Beheshti UniversityTehranIran
  4. 4.Marquette University School of DentistryMilwaukeeUSA
  5. 5.Electrical and Computer Engineering DepartmentNorth Carolina State UniversityRaleighUSA
  6. 6.Biomaterials and Advanced Drug Delivery Laboratory, School of MedicineStanford UniversityPalo AltoUSA
  7. 7.Wellman Center for PhotomedicineMassachusetts General HospitalBostonUSA
  8. 8.Department of DermatologyHarvard Medical SchoolBostonUSA
  9. 9.Harvard-MIT Division of Health Sciences and TechnologyCambridgeUSA

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