Multiplexed microarrays based on optically encoded microbeads
In recent years, there has been growing interest in optically-encoded or tagged functionalized microbeads as a solid support platform to capture proteins or nucleotides which may serve as biomarkers of various diseases. Multiplexing technologies (suspension array or planar array) based on optically encoded microspheres have made possible the observation of relatively minor changes in biomarkers related to specific diseases. The ability to identify these changes at an early stage may allow the diagnosis of serious diseases (e.g. cancer) at a time-point when curative treatment may still be possible. As the overall accuracy of current diagnostic methods for some diseases is often disappointing, multiplexed assays based on optically encoded microbeads could play an important role to detect biomarkers of diseases in a non-invasive and accurate manner. However, detection systems based on functionalized encoded microbeads are still an emerging technology, and more research needs to be done in the future. This review paper is a preliminary attempt to summarize the state-of-the-art concerning diagnostic microbeads; including microsphere composition, synthesis, encoding technology, detection systems, and applications.
KeywordsMicrobeads Optical encoding Multiplexing technologies Early diagnosis
The work was supported by the Faculty of Biomedical Engineering, Amirkabir University of Technology. Michael R Hamblin was supported by US National Institute of Health (NIH) under Grants R01AI050875 and R21AI121700.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- M. Alizadeh, R. Salimi, H. Sameie, A.A. Sarabi, A.A. Sabbagh Alvani, M. Tahriri, The wet-chemical synthesis of functionalized Zn1–xOMnx quantum dots utilizable in optical biosensors. Mater. Technol. 47(2), 235–237 (2013)Google Scholar
- M.M. Babu, Introduction to microarray data analysis. Computational Genomics: Theory and Application, 225–249 (2004)Google Scholar
- R. Breitling, Biological microarray interpretation: the rules of engagement. BBA-Gen Subjects 1759(7), 319–327 (2006)Google Scholar
- Z.A. Dehqanzada et al., Assessing serum cytokine profiles in breast cancer patients receiving a HER2/neu vaccine using Luminex® technology. Oncol. Rep. 17(3), 687–694 (2007)Google Scholar
- G.T. Hermanson, Bioconjugate techniques (Academic Press, Cambridge, 2013)Google Scholar
- D. Jin, Y. Lu, J. Zhao, Multiplex suspension assay/array using lifetime coding. Google Patents (2013)Google Scholar
- O. Khanna et al., Generation of alginate microspheres for biomedical applications. JoVE (Journal of Visualized Experiments) 66, e3388–e3388 (2012)Google Scholar
- G. Ma, Z.-G. Su, Microspheres and microcapsules in biotechnology: design, preparation and applications (CRC Press, Boca Raton, 2013)Google Scholar
- S. Margel, Method for attaching microspheres to a substrate. Google Patents (1997)Google Scholar
- E. Mohagheghpour et al., Effect of manganese (Mn) doping on the optical properties of zinc sulfide (ZnS) semiconductor nanocrystals. J. Ceram. Process. Res. 11(2), 144 (2010)Google Scholar
- E. Mohagheghpour, et al., A new optical bio-sensor: Wet-chemical synthesis and surface treatment of nanocrystalline Zn1-xS: Mn+2x. In Optical Sensors. Optical Society of America. (2011)Google Scholar
- SERVICES, D.O.H.A.H. and F.a.D. Administration, Immucor PreciseType™ Human Erythrocyte Antigen Molecular BeadChip Test. FDA MARyland (2014)Google Scholar
- M. Tayebi et al., Synthesis, surface modification and optical properties of thioglycolic acid-capped ZnS quantum dots for starch recognition at ultralow concentration. J. Electron. Mater., 1–8 (2016a)Google Scholar
- J.A. Thompson, Microbead-based biosensing in microfluidic devices (2011)Google Scholar