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Biomedical Microdevices

, 20:67 | Cite as

A microfluidic device for motility and osmolality analysis of zebrafish sperm

  • Jacob Beckham
  • Faiz Alam
  • Victor Omojola
  • Thomas Scherr
  • Amy Guitreau
  • Adam Melvin
  • Daniel S. Park
  • Jin-Woo Choi
  • Terrence R. Tiersch
  • W. Todd Monroe
Article
  • 97 Downloads

Abstract

A microfluidic chip is described that facilitates research and quality control analysis of zebrafish sperm which, due to its miniscule (i.e., 2–5 μl) sample volume and short duration of motility (i.e., <1 min), present a challenge for traditional manual assessment methods. A micromixer molded in polydimethylsiloxane (PDMS) bonded to a glass substrate was used to activate sperm samples by mixing with water, initiated by the user depressing a transfer pipette connected to the chip. Sample flow in the microfluidic viewing chamber was able to be halted within 1 s, allowing for rapid analysis of the sample using established computer-assisted sperm analysis (CASA) methods. Zebrafish sperm cell activation was consistent with manual hand mixing and yielded higher values of motility at earlier time points, as well as more subtle time-dependent trends in motility, than those processed by hand. Sperm activation curves, which indicate sample quality by evaluating percentage and duration of motility at various solution osmolalities, were generated with on-chip microfabricated gold floor electrodes interrogated by impedance spectroscopy. The magnitude of admittance was linearly proportional to osmolality and was not affected by the presence of sperm cells in the vicinity of the electrodes. This device represents a pivotal step in streamlining methods for consistent, rapid assessment of sperm quality for aquatic species. The capability to rapidly activate sperm and consistently measure motility with CASA using the microfluidic device described herein will help improve the reproducibility of studies on sperm and assist development of germplasm repositories.

Keywords

Zebrafish Sperm Motility Impedance 

Notes

Acknowledgements

We thank L. Torres for assistance with sperm sample preparation, R.L. McCarley for access to equipment, and D.J. Hayes for insightful discussions. We also acknowledge support from the National Institutes of Health grants R24-RR023998, R24-OD011120 and 2R24-OD010441, and the National Institute of Food and Agriculture, United States Department of Agriculture (Hatch project LAB94231 and NC1194). This report was approved for publication by the Director of the Louisiana Agricultural Experiment Station as number 2017-241-31421.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological & Agricultural EngineeringLouisiana State University and Agricultural CenterBaton RougeUSA
  2. 2.Department of ChemistryVanderbilt UniversityNashvilleUSA
  3. 3.Aquatic Germplasm and Genetic Resources CenterLouisiana State University Agricultural CenterBaton RougeUSA
  4. 4.Cain Department of Chemical EngineeringLouisiana State UniversityBaton RougeUSA
  5. 5.Department of Mechanical EngineeringLouisiana State UniversityBaton RougeUSA
  6. 6.School of Electrical Engineering & Computer ScienceLouisiana State UniversityBaton RougeUSA

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