Biomedical Microdevices

, 20:60 | Cite as

A magnetic beads-based portable flow cytometry immunosensor for in-situ detection of marine biotoxin

  • Yuxiang Pan
  • Xinwei Wei
  • Tao Liang
  • Jie Zhou
  • Hao Wan
  • Ning HuEmail author
  • Ping WangEmail author


Okadaic acid (OA), a representative diarrhetic shellfish poisoning toxin, mainly produced by toxigenic dinoflagellates, has significant hazard to public health. Traditional methods for detection of OA can not give the consideration to the need of rapid, high sensitive, quantitative and in-situ detection at the same time. Herein, a new effective detection method of OA was developed based on fluorescence immunosensor and flow cytometry (FCM). In this assay, Streptavidin-coated magnetic beads (MBs) were used as the supporter to immobilize the biotinylated OA. Modified MBs competed with the free OA in the sample solution to bind with the anti-OA monoclonal antibody (OA-MAb). The R-phycoerythrin (R-PE) dye labeled IgG was served as a secondary antibody to perform fluorescence detection. A portable flow cytometry was applied for the in-situ fluorescence quantification. The results showed that the OA concentration was inversely proportional to the R-PE fluorescence intensity. The detection method took within 50 min with a limit of detection (LOD) was 0.05 μg/L and range from 0.2 to 20 μg/L for OA detection. Moreover, the matrix effect and the recovery rate were assessed during real sample measurement, showing a high recovery. Performance features such as high sensitivity, low LOD, speediness and simplicity of the analysis protocol, shows this biosensing-systems as a promising tool for routine use.


Fluorescence immunosensor Portable flow cytometry Magnetic beads-based flow cytometry In-situ detection Okadaic acid 



This work was supported by a key project of the Natural Science Foundation of China (No. 31627801), International Cooperation Project of Natural Science Foundation of China (No. 61320106002,31661143030,), National 973 Project of China (No.2015CB352101), and Natural Science Foundation of China (No. 31571004).


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical EngineeringZhejiang UniversityHangzhouChina
  2. 2.State Key Laboratory of Transducer TechnologyChinese Academy of SciencesShanghaiChina
  3. 3.Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic EngineeringShenzhen UniversityShenzhenChina
  4. 4.Department of Medicine, Biomaterials Innovation Research Center, Center for Biomedical EngineeringBrigham and Women’s Hospital, Harvard Medical SchoolCambridgeUSA

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