An efficient method for CTCs screening with excellent operability by integrating Parsortix™-like cell separation chip and selective size amplification
In this article, an attempt for efficient screening of circulating tumor cells (CTCs) with excellent operability on microfluidic chips was reported. A Parsortix™-like cell separation chip was manufactured in our lab. This chip allowed lateral flow of fluid which increased the flow rate of blood. And, an air valve controlled injection pump was manufactured which allowed eight chips working simultaneously. This greatly facilitated the blood treatment process and saved time. As for the mechanism of screening circulating tumor cells, selective size amplification was utilized. By size amplification of cancer cells, both the hardness and the size of CTCs increased which differentiated them from blood cells. And the modification procedure of beads used for size amplification of cancer cells was optimized. Finally, by integrating the commercialized Parsortix™-like cell separation chip and selective size amplification, a practical method for screening circulating tumor cells was established.
KeywordsCirculating tumor cells Commercialized chips Selective size amplification High through-put Good operability
This research was supported by the National High-tech R&D Program of China (863 Program 2015AA020401), the National Basic Research Program (2015CB931801) and the National Natural Science Foundation of China (51690151, 51473093).
- J. Chen, D. Chen, T. Yuan, Y. Xie, X. Chen, Biomicrofluidics, 7 (2013)Google Scholar
- E. Ozkumur, A.M. Shah, J.C. Ciciliano, B.L. Emmink, D.T. Miyamoto, E. Brachtel, M. Yu, P.I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S.L. Stott, N.M. Karabacak, T.A. Barber, J.R. Walsh, K. Smith, P.S. Spuhler, J.P. Sullivan, R.J. Lee, D.T. Ting, X. Luo, A.T. Shaw, A. Bardia, L.V. Sequist, D.N. Louis, S. Maheswaran, R. Kapur, D.A. Haber, M. Toner, Sci. Transl. Med. 5, 179ra47 (2013)CrossRefGoogle Scholar
- S.L. Stott, C.H. Hsu, D.I. Tsukrov, M. Yu, D.T. Miyamoto, B.A. Waltman, S.M. Rothenberg, A.M. Shah, M.E. Smas, G.K. Korir, F.P. Floyd, A.J. Gilman, J.B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L.V. Sequist, R.J. Lee, K.J. Isselbacher, S. Maheswaran, D.A. Haber, M. Toner, Proc. Natl. Acad. Sci. U. S. A. 107, 18392–18397 (2010)CrossRefGoogle Scholar