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The evaluation of E. faecalis colonies dissolution ability of sodium hypochlorite in microenvironment by a novel device

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Abstract

Enterococcus faecalis(E. faecalis) is a common microorganism could be isolated from the infected canals, especially in the case of refractory apical periodontitis. Due to its ability to invade the dentinal tubules and highly resistant to antimicrobial strategies, the thorough debridement of E.faecalis is hard to achieve. And that may be one of the reasons to cause reinfection and therapeutic failure. According to the anatomy of dentinal tubules published before and the results of our team previous work, we designed six types of microtubes with different sizes. By using the method of centrifugation and incubation, a standard infected model mimicking dentinal tubules was established. Sodium hypochlorite (NaClO) is the most popular irrigant applied in root canal treatment. We used three different concentrations with four distinct irrigation duration to observe the antibacterial process of E. faecalis colonies within microtubes dynamically. We concluded that the role of NaClO in the microtubes is concentration dependent and duration dependent. And the interpretation of the results has a certain reference value for clinicians.

Keywords

Microtube Dentinal tubules Enterococcus faecalis Sodium hypochlorite Dissolution 
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Notes

Acknowledgements

This study was supported by the NSF of China (11674010, 11434001, 81170952) and Science and Technology Project of Dongcheng District, Beijing 2017-3-004.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Department of Endodontics, School of StomatologyCapital Medical UniversityBeijingChina
  2. 2.The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of PhysicsPeking UniversityBeijingChina
  3. 3.Center for Quantitative Biology, Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingChina

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