Algae induce siderophore biosynthesis in the freshwater bacterium Cupriavidus necator H16
- 109 Downloads
Cupriachelin is a photoreactive lipopeptide siderophore produced by the freshwater bacterium Cupriavidus necator H16. In the presence of sunlight, the iron-loaded siderophore undergoes photolytic cleavage, thereby releasing solubilized iron into the environment. This iron is not only available to the siderophore producer, but also to the surrounding microbial community. In this study, the cupriachelin-based interaction between C. necator H16 and the freshwater diatom Navicula pelliculosa was investigated. A reporter strain of the bacterium was constructed to study differential expression levels of the cupriachelin biosynthesis gene cucJ in response to varying environmental conditions. Not only iron starvation, but also culture supernatants of N. pelliculosa were found to induce cupriachelin biosynthesis. The transcription factors involved in this differential gene expression were identified using DNA–protein pulldown assays. Besides the well-characterized ferric uptake regulator, a two-component system was found to tune the expression of cupriachelin biosynthesis genes in the presence of diatom supernatants.
KeywordsSiderophore Cupriavidus necator Diatom Interaction Freshwater
This project was supported by the Collaborative Research Center ChemBioSys (CRC1127 ChemBioSys) and funded by the Deutsche Forschungsgemeinschaft. We thank T. Kindel (Hans Knöll Institute Jena, Department for Molecular and Applied Microbiology) for MALDI-TOF/TOF measurements.
- Bruland KW, Rue EL (2001) Analytical methods for the determination of concentrations and speciation of iron. In: Turner DK, Hunter KA (eds) The biogeochemistry of seawater. Wiley, New YorkGoogle Scholar
- Dean CR, Poole K (1993) Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas aeruginosa: involvement of a two-component regulatory system. Mol Microbiol 8:1095–1103. https://doi.org/10.1111/j.1365-2958.1993.tb01654.x CrossRefGoogle Scholar
- Ferrero M, Farías ME, Siñeriz F (2004) Preliminary characterization of microbial communities in high altitude wetlands of northwestern Argentina by determining terminal restriction fragment length polymorphisms. Rev Latinoam Microbiol 46:72–80Google Scholar
- Kohlmann Y (2015) Charakterisierung des Proteoms von Ralstonia eutropha H16 unter lithoautotrophen und anaeroben Bedingungen. Humboldt University of Berlin, BerlinGoogle Scholar
- Lowry R (2018) VassarStats: website for statistical computation. Vassar College, PoughkeepsieGoogle Scholar
- Schalk IJ, Guillon L (2013) Fate of ferrisiderophores after import across bacterial outer membranes: different iron release strategies are observed in the cytoplasm or periplasm depending on the siderophore pathways. Amino Acids 44:1267–1277. https://doi.org/10.1007/s00726-013-1468-2 CrossRefGoogle Scholar
- Stintzi A, Evans K, Meyer J, Poole K (1998) Quorum-sensing and siderophore biosynthesis in Pseudomonas aeruginosa: lasRllasI mutants exhibit reduced pyoverdine biosynthesis. FEMS Microbiol Lett 166:341–345. https://doi.org/10.1111/j.1574-6968.1998.tb13910.x CrossRefGoogle Scholar
- Swanepoel A (2015) Sampling of diatoms and bacteria from the epilithic biofilm. In: T&M conference proceedings, M104Google Scholar