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BioMetals

, Volume 32, Issue 1, pp 65–76 | Cite as

Arsenite biotransformation and bioaccumulation by Klebsiella pneumoniae strain SSSW7 possessing arsenite oxidase (aioA) gene

  • Sajiya Yusuf Mujawar
  • Kashif Shamim
  • Diviya Chandrakant Vaigankar
  • Santosh Kumar DubeyEmail author
Article
  • 74 Downloads

Abstract

Arsenite oxidizing Klebsiella pneumoniae strain SSSW7 isolated from shipyard waste Goa, India showed a minimum inhibitory concentration of 21 mM in mineral salts medium. The strain possessed a small supercoiled plasmid and PCR amplification of arsenite oxidase gene (aioA) was observed on plasmid as well as chromosomal DNA. It was confirmed that arsenite oxidase enzyme was a periplasmic protein with a 47% increase in arsenite oxidase activity at 1 mM sodium arsenite. Scanning electron microscopy coupled with electron dispersive X-ray spectroscopic (SEM–EDS) analysis of 15 mM arsenite exposed cells revealed long chains of cells with no surface adsorption of arsenic. Transmission electron microscopy combined with electron dispersive X-ray spectroscopic (TEM-EDS) analysis demonstrated plasma membrane disruption, cytoplasmic condensation and periplasmic accumulation of arsenic. The bacterial strain oxidized 10 mM of highly toxic arsenite to less toxic arsenate after 24 h of incubation. Fourier transformed infrared (FTIR) spectroscopy confirmed the interaction of arsenite with functional groups present on the bacterial cell surface. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of 5 mM arsenite exposed cells demonstrated over-expression of 87 kDa and 14 kDa proteins of two subunits aioA and aioB of heterodimer arsenite oxidase enzyme as compared to control cells. Therefore, this bacterial strain might be employed as a potential candidate for bioremediation of arsenite contaminated environmental sites.

Keywords

Arsenite AioA gene Bioremediation Biotransformation 

Notes

Acknowledgements

SM is grateful to University Grants Commission, New Delhi for financial support as Maulana Azad National Fellowship (SRF). The authors are thankful to Areef Sardar from CSIR- National Institute of Oceanography, Goa for EDX analysis; AIRF, Jawaharlal Nehru University, New Delhi for TEM–EDX analysis; B. R. Srinivasan, Head, Department of Chemistry and Rahul Kerkar from Department of Chemistry, Goa University for FTIR analysis. SM is also thankful to Sandeep Garg, Head, Department of Microbiology, Goa University for providing laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

10534_2018_158_MOESM1_ESM.docx (224 kb)
Supplementary material 1 (DOCX 224 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Sajiya Yusuf Mujawar
    • 1
  • Kashif Shamim
    • 1
  • Diviya Chandrakant Vaigankar
    • 1
  • Santosh Kumar Dubey
    • 2
    Email author
  1. 1.Laboratory of Bacterial Genetics and Environmental Biotechnology, Department of MicrobiologyGoa UniversityTaleigao PlateauIndia
  2. 2.Center of Advanced Study in BotanyBanaras Hindu UniversityVaranasiIndia

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