, Volume 140, Issue 1, pp 15–30 | Cite as

Influence of pH and redox on mobilization of inositol hexakisphosphate from oligotrophic lake sediment

  • Kasper ReitzelEmail author
  • Henning S. Jensen
  • Benjamin L. Turner
  • Charlotte Jørgensen


It has been suggested that inositol hexakisphosphate (IP6) contributes to the release of phosphorus (P) from lake sediments, but a mechanistic understanding remains elusive. We investigated the potential mobilization and mineralization of myo- and scyllo-IP6 from the sediment of an oligotrophic Danish lake known to contain high concentrations of inositol phosphates. Solution 31P NMR spectroscopy was used to determine changes in myo- and scyllo-IP6 in laboratory microcosms incubated under either oxic or anoxic conditions. In addition, we incubated sediment slurries adjusted to pH between 4.9 and 6.6, with and without addition of myo-IP6, and induced redox changes by adding starch and sulfate. We observed no significant changes in myo- or scyllo-IP6 after 1 year of incubation under anaerobic conditions. A sequential extraction procedure revealed that one half of the added myo-IP6 was recovered in the humic acid fraction (acid-insoluble organic matter) and the other half in the fulvic acid fraction (acid-soluble organic matter). Reduction in redox potential by starch addition did not mobilize myo-IP6, but myo-IP6 bound to humic acids was released to the pore water when the pH was increased to ≥ 5.8. This pH-induced mobilization of IP6 occurred in parallel with increases in dissolved iron and organic matter, suggesting the release of IP6 bound to humic acids through metal bridges. We conclude that myo-IP6 mobilization from this oligotrophic lake sediment is driven by changes in pH rather than by changes in the redox potential.


myo-inositol hexakisphosphate scyllo-inositol hexakisphosphate d-chiro-inositol hexakisphosphate neo-inositol hexakisphosphate Solution 31P NMR spectroscopy Sequential phosphorus extraction 



We thank the Villum Kann Rasmussen Foundation for financial support to the Centre of Excellence CLEAR (Centre for Lake Restoration).

Supplementary material

10533_2018_468_MOESM1_ESM.docx (52 kb)
Supplementary material 1 (DOCX 51 kb)


  1. Ahlgren J, Tranvik L, Gogoll A et al (2005) Sediment depth attenuation of biogenic phosphorus compounds measured by 31P NMR. Environ Sci Technol 39:867–872CrossRefGoogle Scholar
  2. Ahlgren J, De Brabandere H, Reitzel K et al (2007) Sediment phosphorus extractants for phosphorus-31 nuclear magnetic resonance analysis: a quantitative evaluation. J Environ Qual 36:892–898CrossRefGoogle Scholar
  3. Boström B, Andersen JM, Fleischer S et al (1988) Exchange of phosphorus across the sediment-water interface. In: Phosphorus in freshwater ecosystems. Springer, New York, pp 229–244CrossRefGoogle Scholar
  4. Caraco NF, Cole JJ, Likens GE (1989) Evidence for sulfate-controlled phosphorus release from sediments of aquatic systems. Nature 341:316–318CrossRefGoogle Scholar
  5. Celi L, Barberis E (2007) Abiotic reactions of inositol phosphates in soil. In: Turner BL et al (eds) Inositol phosphates: linking agriculture and the environment. CABI Publishing, Wallingford, pp 207–220CrossRefGoogle Scholar
  6. Celi L, Lamacchia S, Marsan FA et al (1999) Interaction of inositol hexaphosphate on clays: adsorption and charging phenomena. Soil Sci 164:574–585CrossRefGoogle Scholar
  7. Celi L, Presta M, Ajmone-Marsan F et al (2001) Effects of pH and electrolytes on inositol hexaphosphate interaction with goethite. Soil Sci Soc Am J 65:753–760CrossRefGoogle Scholar
  8. Celi L, De Luca G, Barberis E (2003) Effects of interaction of organic and inorganic P with ferrihydrite and kaolinite-iron oxide systems on iron release. Soil Sci 168:479–488Google Scholar
  9. Christensen KK (1999) Comparison of iron and phosphorus mobilization from sediments inhabited by Littorella uniflora and Sphagnum sp. at different sulfate concentrations. Arch Hydrobiol 145:257–275CrossRefGoogle Scholar
  10. Einsele W (1938) Uber chemische und kolloidchemische vorgange in eisen-phosphat-systemen unter limnochemischen und limnogeologischen gesichtspunkten. Arch Hydrobiol 33:361–387Google Scholar
  11. Gardolinski PC, Worsfold PJ, McKelvie ID (2004) Seawater induced release and transformation of organic and inorganic phosphorus from river sediments. Water Res 38:688–692CrossRefGoogle Scholar
  12. Gibbs MM (1979) A simple method for the rapid determination of iron in natural waters. Water Res 13:295–297CrossRefGoogle Scholar
  13. Golterman H, Paing J, Serrano L et al (1998) Presence of and phosphate release from polyphosphates or phytate phosphate in lake sediments. Hydrobiologia 364:99–104CrossRefGoogle Scholar
  14. Guan XH, Shang C, Zhu J et al (2006) ATR-FTIR investigation on the complexation of myo-inositol hexaphosphate with aluminum hydroxide. J Colloid Interface Sci 293:296–302CrossRefGoogle Scholar
  15. Hansen JW, Thamdrup B, Jorgensen BB (2000) Anoxic incubation of sediment in gas-tight plastic bags: a method for biogeochemical process studies. Mar Ecol Prog Ser 208:273–282CrossRefGoogle Scholar
  16. He Z, Dao T, Honeycutt CW (2006) Insoluble Fe-associated inorganic and organic phosphates in animal manure and soil. Soil Sci 171:117–126CrossRefGoogle Scholar
  17. Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70Google Scholar
  18. Hongve D, Akesson G (1996) Spectrophotometric determination of water color in Hazen units. Water Res 30:2771–2775CrossRefGoogle Scholar
  19. Hupfer M, Lewandowski J (2008) Oxygen controls the phosphorus release from lake sediments—a long-lasting paradigm in limnology. Int Rev Hydrobiol 93:415–432CrossRefGoogle Scholar
  20. Hupfer M, Herzog C, Schmieder P (2010) Sedimentation and diagenetic transformation of particulate organic phosphorus in a stratified eutrophic lake. Verh Int Verein Limnol 30:1389–1392Google Scholar
  21. Jensen HS, Caraco N, Hansen J et al (2005) Humic-bound phosphorus in soil and sediment. In: Serano L, Golterman HL (eds) Phosphates in sediments. Backhuys Publishers, Leiden, pp 99–107Google Scholar
  22. Jørgensen C, Jensen HS, Andersen FO et al (2011) Occurrence of orthophosphate monoesters in lake sediments: significance of myo- and scyllo-inositol hexakisphosphate. J Environ Monitor 13:2328–2334CrossRefGoogle Scholar
  23. Klamt A-M, Jensen HS, Mortensen MF et al (2017) The importance of catchment vegetation for alkalinity, phosphorus burial and macrophytes as revealed by a recent paleolimnological study in a soft water lake. Sci Total Environ 580:1097–1107CrossRefGoogle Scholar
  24. Koroleff F (1983) Determination of total nutrients. In: Grasshoff K et al (eds) Methods for seawater analysis. Verl Chem, Weinheim, pp 125–131Google Scholar
  25. Leytem AB, Maguire RO (2007) Environmental implications of animal manures. In: Turner BL et al (eds) Inositol phosphates: linking agriculture and the environment. CABI Publishing, Wallingford, pp 261–279Google Scholar
  26. Liu SS, Zhu YR, Meng W et al (2016) Characteristics and degradation of carbon and phosphorus from aquatic macrophytes in lakes: insights from solid-state 13C NMR and solution 31P NMR spectroscopy. Sci Total Environ 543:746–756CrossRefGoogle Scholar
  27. Martin M, Celi L, Barberis E (2004) Desorption and plant availability of myo-inositol hexaphosphate adsorbed on goethite. Soil Sci 169:115–124CrossRefGoogle Scholar
  28. McKelvie ID (2007) Inositol phosphates in aquatic systems. In: Turner BL et al (eds) Inositol phosphates: linking agriculture and the environment. CABI Publishing, Wallingford, pp 261–279CrossRefGoogle Scholar
  29. Monbet P, McKelvie ID, Worsfold PJ (2009) Dissolved organic phosphorus speciation in the waters of the Tamar eustuary (SW England). Geochim Cosmochim Acta 73:1027–1038CrossRefGoogle Scholar
  30. Paludan C, Jensen HS (1995) Sequential extraction of phosphorus in freshwater wetland and lake sediment: significance of humic acids. Wetlands 15:365–373CrossRefGoogle Scholar
  31. Psenner PR, Pucsko R, Sager M (1984) Die fractionerung organisher und anorganisher phosphorverbindungen von sedimenten. Arch Hydrobiol 70:111–155Google Scholar
  32. Reitzel K (2005) Separation of aluminum bound phosphate from iron bound phosphate in freshwater sediments by a sequential extraction procedure. In: Serano L, Golterman HL (eds) Phosphates in sediments. Backhuys Publishers, Leiden, pp 109–117Google Scholar
  33. Reitzel K, Hansen J, Andersen FO et al (2005) Lake restoration by dosing aluminum relative to mobile phosphorus in the sediment. Environ Sci Technol 39:4134–4140CrossRefGoogle Scholar
  34. Reitzel K, Ahlgren J, Gogoll A et al (2006) Characterization of phosphorus in sequential extracts from lake sediments using 31P nuclear magnetic resonance spectroscopy. Can J Fish Aquat Sci 63:1686–1699CrossRefGoogle Scholar
  35. Reitzel K, Ahlgren J, DeBrabandere H et al (2007) Degradation rates of organic phosphorus in lake sediment. Biogeochemistry 82:15–28CrossRefGoogle Scholar
  36. Reitzel K, Balslev KA, Jensen HS (2017) The influence of lake water alkalinity and humic substances on particle dispersion and lanthanum desorption from a Lanthanum modified bentonite. Water Res 125:191–200CrossRefGoogle Scholar
  37. Roden EE, Edmonds JW (1997) Phosphate mobilization in iron-rich anaerobic sediments: microbial Fe(III) oxide reduction versus iron-sulfide formation. Arch Hydrobiol 139:347–378Google Scholar
  38. Suzumura M, Kamatani A (1993) Isolation and determination of inositol hexaphosphate in sediments from Tokyo Bay. Geochim Cosmochim Acta 57:2197–2202CrossRefGoogle Scholar
  39. Suzumura M, Kamatani A (1995a) Mineralization of inositol hexaphosphate in aerobic and anaerobic marine sediments—implications for the phosphorus cycle. Geochim Cosmochim Acta 59:1021–1026CrossRefGoogle Scholar
  40. Suzumura M, Kamatani A (1995b) Origin and distribution of inositol hexaphosphate in estuarine and coastal sediments. Limnol Oceanogr 40:1254–1261CrossRefGoogle Scholar
  41. Turner BL (2007) Inositol phosphates in soil: amounts, forms and significance of the phosphorlyated inositol stereoisomers. In: Turner BL et al (eds) Inositol phosphates: linking agriculture and the environment. CAB International, Wallingford, pp 186–207CrossRefGoogle Scholar
  42. Turner BL, Weckström K (2009) Phytate as a novel phosphorus-specific paleo-indicator in aquatic sediments. J Paleolimnol 42:391–400CrossRefGoogle Scholar
  43. Turner BL, Paphazy MJ, Haygarth PM et al (2002) Inositol phosphates in the environment. Philos Trans R Soc Lond B 357:449–469CrossRefGoogle Scholar
  44. Turner BL, Mahieu N, Condron LM (2003) Quantification of myo-inositol hexakisphosphate in alkaline soil extracts by solution 31P NMR spectroscopy and spectral deconvolution. Soil Sci 168:469–478Google Scholar
  45. Turner BL, Cheesman AW, Godage HY et al (2012) Determination of neo- and d-chiro-inositol hexakisphosphate in soils by solution 31P NMR spectroscopy. Environ Sci Technol 46:4994–5002CrossRefGoogle Scholar
  46. Vestergren J, Vincent AG, Jansson M et al (2012) High-resolution characterization of organic phosphorus in soil extracts using 2D 1H–31P NMR correlation spectroscopy. Environ Sci Technol 46:3950–3956CrossRefGoogle Scholar
  47. Wetzel RG (2001) Limnology: lake and river ecosystems, 3rd edn. Elsevier Science, San Diego, pp 187–205CrossRefGoogle Scholar
  48. Xiong JB, Mahmood Q (2010) Adsorptive removal of phosphate from aqueous media by peat. Desalination 259:59–64CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Kasper Reitzel
    • 1
    Email author
  • Henning S. Jensen
    • 1
  • Benjamin L. Turner
    • 2
  • Charlotte Jørgensen
    • 1
  1. 1.Institute of Biology, University of Southern DenmarkOdense MDenmark
  2. 2.Smithsonian Tropical Research InstituteBalboa, AnconPanama

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