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Evaluation of methanotrophic bacterial communities capable of biodegrading trichloroethene (TCE) in acidic aquifers

  • Yiru Shao
  • Paul B. HatzingerEmail author
  • Sheryl H. Streger
  • Rachael T. Rezes
  • Kung-Hui Chu
Original Paper
  • 6 Downloads

Abstract

While bioremediation technologies for trichloroethene (TCE), a suspected carcinogen, have been successfully demonstrated in neutral pH aquifers, these technologies are often ineffective for remediating TCE contamination in acidic aquifers (i.e., pH < 5.5). Acidophilic methanotrophs have been detected in several low pH environments, but their presence and potential role in TCE degradation in acidic aquifers is unknown. This study applied a stable isotope probing-based technique to identify active methanotrophs that are capable of degrading TCE in microcosms prepared from two low pH aquifers. A total of thirty-five clones of methanotrophs were derived from low pH microcosms in which methane and TCE degradation had been observed, with 29 clustered in γ-Proteobacteria and 6 clustered in α-Proteobacteria. None of the clones has a high similarity to known acidophilic methanotrophs from other environments. The presence and diversity of particulate MMO and soluble MMO were also investigated. The pmoA gene was detected predominantly at one site, and the presence of a specific form of mmoX in numerous samples suggested that Methylocella spp. may be common in acidic aquifers. Finally, a methane-grown culture at pH 4 was enriched from an acidic aquifer and its ability to biodegrade various chlorinated ethenes was tested. Interestingly, the mixed culture rapidly degraded TCE and vinyl chloride, but not cis-dichloroethene after growth on methane. The data suggest that aerobic biodegradation of TCE and other chlorinated solvents in low pH groundwater may be facilitated by methanotrophic bacteria, and that there are potentially a wide variety of different strains that inhabit acidic aquifers.

Graphical abstract

Keywords

Acidophilic methanotrophs Low pH Stable-isotope-probing (SIP) MMO 

Notes

Acknowledgements

The authors thank the Strategic Environmental Research and Development Program (SERDP; Project ER-2531) for supporting some of the studies described herein as well as anonymous reviewers for their helpful suggestions. The results and interpretations presented are solely the opinion of the authors and not of SERDP unless otherwise stated in official documentation.

Supplementary material

10532_2019_9875_MOESM1_ESM.pdf (209 kb)
Supplementary material 1 (PDF 235 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yiru Shao
    • 1
  • Paul B. Hatzinger
    • 2
    Email author
  • Sheryl H. Streger
    • 2
  • Rachael T. Rezes
    • 2
  • Kung-Hui Chu
    • 1
  1. 1.Zachry Department of Civil EngineeringTexas A&M UniversityCollege StationUSA
  2. 2.APTIMLawrencevilleUSA

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