Simultaneous production of alkaline amylase and biosurfactant by Bacillus methylotrophicus DCS1: application as detergent additive
- 122 Downloads
This study investigated the coproduction of alkaline amylase and lipopeptides by Bacillus methylotrophicus DCS1 strain, as well as their biochemical characterisation. The best production of both amylase and biosurfactant was obtained when potato starch (10 g/L) and glutamic acid (5 g/L) were used as carbon and nitrogen sources, respectively. The bacterial strain was incubated for 48 h at 25 °C and 150 rpm. This strain produced a unique amylase as showed by zymography technique. The optima pH and temperature were 60–65 °C and 8.0, respectively. Amylase activity was partially inhibited by EDTA (5 mM). The main hydrolysis products of potato starch were maltose and maltotriose. The alkaline amylase showed excellent stability and compatibility with various solid and liquid detergents. Furthermore, the biosurfactant, produced simultaneously with alkaline amylase, demonstrated high stability at different ranges of salinity, pH, and temperature. Considering its promising properties, B. methylotrophicus DCS1 crude extract containing both biosurfactants and amylase activity may be considered as a potential candidate for future use in detergent processing industries and environmental remediation processes.
Keywordsα-Amylase Bacillus methylotrophicus Biosurfactant Biochemical characterization Detergent
This work was funded by «Ministry of Higher Education and Scientific Research-Tunisia».
- Behel A, Singh MK, Sharma MK, Puri P, Batra N (2006) Characterization of alkaline α-amylase from Bacillus sp. AB 04. Int J Agric Biol 8:80–83Google Scholar
- Chen YC, Chiang TJ, Liang TW, Wang IL, Wang SL (2012) Reclamation of squid pen by Bacillus licheniformis TKU004 for the production of thermally stable and antimicrobial biosurfactant. Biocatal Agric Biotechnol 1:62–69Google Scholar
- Dahiya P, Rathi Amity RB (2015) Characterization and application of alkaline α-amylase from Bacillus licheniformis MTCC1483 as a detergent additive. Int Food Res J 22:1293–1297Google Scholar
- Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Rev 61:47–64Google Scholar
- Luna JM, Rufino RD, Campos-Takakia GM, Sarubbo LA (2012) Properties of the biosurfactant produced by Candida sphaerica cultivated in low-cost substrates. Chem Eng Trans 27:67–72Google Scholar
- Miller JH (1972) Experiments in moleculer genetics. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Mukherjee AK, Borah M, Rai SK (2009) To study the influence of different components of fermentable substrates on induction of extracellular a-amylase synthesis by Bacillus subtilis DM-03 in solid-state fermentation and exploration of feasibility for inclusion of α-amylase in laundry detergent formulations. Biochem Eng J 43:149–156CrossRefGoogle Scholar
- Nusrat A, Rahman SR (2007) Comparative studies on the production of extracellular amylase by three mesophilic Bacillus isolates. Bangladesh J Microbiol 24:129–132Google Scholar
- Roohi R, Kuddus M, Saima S (2013) Cold-active detergent-stable extracellular α-amylase from Bacillus cereus GA6: biochemical characteristics and its perspectives in laundry detergent formulation. J Biochemical Technol 4:636–644Google Scholar
- Sobrinho HB, Luna JM, Rufino RD, Porto ALF, Sarubbo LA (2013) Biosurfactants: classification, properties and environmental applications. In: Govil JN (ed) Recent developments in biotechnology. Studium Press LLC, Houston, pp 1–29Google Scholar
- Wang Q, Chen S, Zhang J, Sun M, Liu Z, Ziniu Y (2008) Co-producing lipopeptides and poly-Î³-glutamic acid by solid-state fermentation of Bacillus subtilis using soybean and sweet potato residues and its biocontrol and fertilizer synergistic effects. Bioresour Technol 99:3318–3323CrossRefPubMedGoogle Scholar