Overproduction of rhamnolipid by fed-batch cultivation of Pseudomonas aeruginosa in a lab-scale fermenter under tight DO control

  • Shayesteh Bazsefidpar
  • Babak MokhtaraniEmail author
  • Reza Panahi
  • Hamidreza Hajfarajollah
Original Paper


Rhamnolipids are one of the most well-known classes of biosurfactants having wide applications in various industries due to low toxicity, high biodegradability, and environmentally friendly. Dissolved oxygen (DO) concentration has the crucial effect on rhamnolipids production, particularly through fed-batch cultivation. In this study, the effect of different levels of DO concentrations on rhamnolipid production by Pseudomonas aeruginosa in both batch and fed-batch fermentation was investigated in a lab-scale fermenter under precise DO control. A maximal rhamnolipid production of 22.5 g/l was obtained at a DO concentration of 40% in batch fermentation. In order to achieve the high rhamnolipid production, a fed-batch operation under tight DO control of 40% was conducted. As a result, the overall rhamnolipid production and productivity reached to 240 g/l and 0.9 (g/l h), corresponding to a 10.7 and 4.8-fold improvement compared to the batch experiments. The high level of rhamnolipid production via the fed-batch cultivation can be attributed to both DO concentration and the feeding strategy. This achievement is promising for the production of rhamnolipid in industrial scale.


Rhamnolipid Biosurfactant Pseudomonas aeruginosa Dissolved oxygen Fed-batch fermentation Productivity 

Supplementary material

10532_2018_9866_MOESM1_ESM.docx (1.6 mb)
Supplementary material 1 (DOCX 1649 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Shayesteh Bazsefidpar
    • 1
  • Babak Mokhtarani
    • 1
    Email author
  • Reza Panahi
    • 1
  • Hamidreza Hajfarajollah
    • 1
  1. 1.Chemistry and Chemical Engineering Research Center of IranTehranIran

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