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A novel huperzine A-producing endophytic fungus Fusarium sp. Rsp5.2 isolated from Huperzia serrate


The isolation, identification and characterization of a novel huperzine A (HupA)-producing fungal strain Rsp5.2 isolated from Huperzia serrata (Thunb. ex Murray) Trev. in Vietnam. The fifty-eight endophytic fungi were recovered from roots of natural H. serrata in Lao Cai province of northern Vietnam and screened for HupA-producing by thin-layer chromatography (TLC). The only one of the 58 strains, Rsp5.2, could produce HupA. The amount of HupA produced by Rsp5.2 was quantified to be 19.45 µg g−1 dried mycelium by high-performance liquid chromatography (HPLC). Acetylcholine esterase (AchE) inhibition (IC50) of the crude HupA extract from Rsp5.2 fermentation broth was 2.849 ± 0.0026 µg mL−1. The fungus was identified as Fusarium sp. Rsp5.2 by morphological characteristics and Internal Transcribed Spacer (ITS) sequences. This is the first report of Fusarium sp. as a HupA-producing endophyte isolated from H. serrata.

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This work was supported by KC.10/16-20 program, Office of National Science and Technology Research Programs, Ministry of Science and Technology, Vietnam (Grand No. KC.10.01/16-20).

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Correspondence to Thanh Thi Minh Le.

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Le, T.T.M., Hoang, A.T.H., Nguyen, N.P. et al. A novel huperzine A-producing endophytic fungus Fusarium sp. Rsp5.2 isolated from Huperzia serrate. Biotechnol Lett (2020). https://doi.org/10.1007/s10529-020-02836-x

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  • Huperzia serrata
  • Huperzine A
  • Endophytic fungi
  • Fusarium sp. Rsp5.2
  • Alzheimer disease