Ac25 in Autographa californica multiple nucleopolyhedrovirus was crucial for progeny budded virion production
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To analyze the effect of Ac25 on the proliferation of AcMNPV (Autographa californica multicapsid nucleopolyhedrovirus) progeny virus and its function in virogenic stroma.
AcMNPV is a model of baculovirus and is the most widely studied baculovirus. Ac25, as a single-stranded DNA-binding protein, is involved in viral genomic DNA replication. Viral proliferation assay showed that AcMNPV progeny virus could not be produced when Ac25 was knocked out, which indicated it was crucial for BV production. Absolute quantitative PCR analysis indicated that Ac25 was able to promote replication of the AcMNPV genome in host Sf9 cells. It was also found that Ac25 could increase the transcription level of 38k and vp39 late expression genes, and inhibit host cell proliferation.
Ac25 is highly accumulated in the nucleus and promotes progeny virus production by stimulating viral genome replication and up-regulating the expression of late genes. Two potential applications of vAc-Ac25-EGFP were proposed: an improved bac-to-bac eukaryotic protein expression systems and biopesticides.
KeywordsAutographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) Spodoptera frugiperda 9 cell Ac25 Biopesticide
The present work was supported by grants from National Natural Science Foundation of China (No. 31272100) and Natural Science Foundation of Shanxi Province (No. 201801D121193) to YJ Fu. It was also supported by grants from Shanxi ‘1331 project’ Collaborative Innovation Center (1331 CIC) and Higher Education Institution Project of Shanxi Province: Ecological Remediation of Soil Pollution Disciplines Group (No. 20181401) to Y. Fu.
- Mikhailov VS, Mikhailova AL, Iwanaga M, Gomi S, Maeda S (1998) Bombyx mori nucleopolyhedrovirus encodes a DNA-binding protein capable of destabilizing duplex DNA. J Virol 72:3107–3116Google Scholar
- Okano K, Mikhailov VS, Maeda S (1999) Colocalization of baculovirus IE-1 and two DNA-binding proteins, DBP and LEF-3, to viral replication factories. J Virol 73:110–119Google Scholar
- Rohrmann GF (2013) Baculovirus molecular biology. National Library of Medicine, National Center for Biotechology Information, BethesdaGoogle Scholar