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Biotechnology Letters

, Volume 41, Issue 10, pp 1163–1175 | Cite as

Kinetics and thermodynamics of lipase catalysed synthesis of propyl caprate

  • Dhara T. Parikh
  • Kavita J. Lanjekar
  • Virendra K. RathodEmail author
Original Research Paper
  • 42 Downloads

Abstract

Objective

To investigate kinetics and thermodynamics of lipase-catalyzed esterification of capric acid with 1-propyl alcohol in a solvent-free system for synthesis of propyl caprate.

Results

The capric acid conversion of 83.82% is achieved at temperature 60 °C, speed of agitation 300 rpm, molar ratio acid:alcohol 1:3, enzyme loading 2% (w/w) and molecular sieves loading 5% (w/w). The activation energy (Ea) for the reaction was determined as 37.79 kJ mol−1. Furthermore, enthalpy (ΔH), entropy (ΔS) and Gibbs free energy (ΔG) values were found out to be + 90.45 kJ mol−1, + 278.99 J mol−1 K−1 and − 2.35 kJ mol−1 respectively.

Conclusions

The results showed that the lipase-catalyzed esterification exhibits an ordered bi–bi mechanism with capric acid inhibiting the reaction and forming the dead-end complex with the lipase. Under the given set of reaction conditions, the lipase catalysed esterification reaction was anticipated to be spontaneous, referring to the value of the Gibbs free energy change (ΔG). Moreover, the esterification process was found to be endothermic, based on the values of enthalpy (ΔH) and entropy (ΔS).

Keywords

Enzyme kinetics Lipase Ordered bi–bi mechanism Propyl caprate Thermodynamics 

Notes

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Dhara T. Parikh
    • 1
  • Kavita J. Lanjekar
    • 1
  • Virendra K. Rathod
    • 1
    Email author
  1. 1.Department of Chemical EngineeringInstitute of Chemical TechnologyMumbaiIndia

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