Preincubation with a low concentration of methyl-β-cyclodextrin enhances baculovirus expression system productivity
- 82 Downloads
To enhance the productivity of foreign protein in culture cells using baculovirus expression system.
A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.
Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.
KeywordsAcMNPV BmNPV Expression Methyl-β-cyclodextrin
This study was funded by the National Science Foundation of China (Grant No. 31670152); and the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (Grant No. 2015).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Chaabihi H, Ogliastro MH, Martin M, Giraud C, Devauchelle G, Cerutti M (1993) Competition between baculovirus polyhedrin and p10 gene expression during infection of insect cells. J Virol 67:2664–2671Google Scholar
- Dong S, Wang M, Qiu Z, Deng F, Vlak JM, Hu Z, Wang H (2010) Autographa californica multicapsid nucleopolyhedrovirus efficiently infects Sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low pH. J Virol 84:5351–5359. https://doi.org/10.1128/jvi.02517-09 CrossRefGoogle Scholar
- Grimmer S, van Deurs B, Sandvig K (2002) Membrane ruffling and macropinocytosis in A431 cells require cholesterol. J Cell Sci 115:2953–2962Google Scholar
- Luckow VA, Lee SC, Barry GF, Olins PO (1993) Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol 67:4566–4579Google Scholar
- Rohrmann GF (2013) Baculovirus molecular biology. National Center for Biotechnology Information, Bethesda (MD)Google Scholar