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Preincubation with a low concentration of methyl-β-cyclodextrin enhances baculovirus expression system productivity

  • Jinshan Huang
  • Na Liu
  • Xingjia Shen
  • Bifang HaoEmail author
Original Research Paper
  • 82 Downloads

Abstract

Objectives

To enhance the productivity of foreign protein in culture cells using baculovirus expression system.

Results

A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.

Conclusions

Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.

Keywords

AcMNPV BmNPV Expression Methyl-β-cyclodextrin 

Notes

Acknowledgements

This study was funded by the National Science Foundation of China (Grant No. 31670152); and the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (Grant No. 2015).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Jinshan Huang
    • 1
    • 2
  • Na Liu
    • 1
  • Xingjia Shen
    • 1
    • 2
  • Bifang Hao
    • 1
    • 2
    Email author
  1. 1.Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangPeople’s Republic of China
  2. 2.Key Laboratory of Genetic Improvement of Sericulture in the Ministry of Agriculture, Sericultural Research InstituteChinese Academy of Agricultural ScienceZhenjiangPeople’s Republic of China

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