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Biotechnology Letters

, Volume 41, Issue 1, pp 91–106 | Cite as

Atheroprotective effect of novel peptides from Porphyridium purpureum in RAW 264.7 macrophage cell line and its molecular docking study

  • Mysore Doddaiah Kavitha
  • Karekal Girinur Mallikarjun Gouda
  • Shimoga Janakirama Aditya Rao
  • Talkad Shivashankara Shilpa
  • Nandini Prasad Shetty
  • Ravi Sarada
Original Research Paper
  • 70 Downloads

Abstract

Objective

To explore the atherogenic foam cell prevention efficiency of two dipeptides purified from Porphyridium purpureum on RAW 264.7 cell line and to study its molecular interaction through molecular docking.

Result

P. purpureum consists of 29.9% protein and 2.98% phycoerythrin on a dry weight basis. The two dipeptides namely of Histidine–Glutamic acid (HE) and Glycine–Proline (GP) isolated from the total protein and purified phycoerythrin of P. purpureum respectively, were evaluated for atherogenic foam cell prevention capacity in RAW 264.7 cell line. The IC5O values of peptides were found to be 91.2 ± 1.81 µg/ml (GP), 103.3 ± 4.8 µg/ml (HE) in MTT assay. The two peptides reduce the foam cell formation, intracellular lipid accumulation (cholesterol and triglycerides) and the secretion of TNF-α and IL-6 which are inflammatory cytokines in RAW 264.7 cell line at non-cytotoxic concentrations. A molecular interaction study proposed the binding pose for GP and HE peptides targeting the scavenging receptors CD36, SRA1, and Map Kinase p38 (a protein mediator).

Conclusions

The cell line and molecular docking study indicated that among the two dipeptides, peptide GP has the highest atherogenic foam cell prevention efficiency.

Keywords

Atherosclerosis Docking Low-density lipoprotein Macrophages Peptides Phycoerythrin Porphyridium purpureum 

Notes

Acknowledgement

The authors thank Director, CSIR-CFTRI for providing facilities to carry out the study. MDK acknowledge the ICMR, for research fellowship grants. Authors thank Dr. Uma Manjappara for her support in synthetic peptide synthesis.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Mysore Doddaiah Kavitha
    • 1
  • Karekal Girinur Mallikarjun Gouda
    • 1
  • Shimoga Janakirama Aditya Rao
    • 2
  • Talkad Shivashankara Shilpa
    • 3
  • Nandini Prasad Shetty
    • 1
  • Ravi Sarada
    • 1
  1. 1.Department of Plant Cell BiotechnologyCentral Food Technological Research InstituteMysoreIndia
  2. 2.Molecular Biomedicine Laboratory, Department of P.G. Studies and Research in Biotechnology, Sahyadri Science College (Autonomous)Kuvempu UniversityShimogaIndia
  3. 3.Department of LipidomicsCentral Food Technological Research InstituteMysoreIndia

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