Advertisement

Biotechnology Letters

, Volume 41, Issue 1, pp 27–34 | Cite as

Advances in heterologous biosynthesis of plant and fungal natural products by modular co-culture engineering

  • Tingting Chen
  • Yiyao Zhou
  • Yinghua Lu
  • Haoran ZhangEmail author
Review
  • 221 Downloads

Abstract

Heterologous biosynthesis has been long pursued as a viable approach for high efficiency production of natural products with various industrial values. Conventional methods for heterologous biosynthesis use the mono-culture of an engineered microbe for accommodating the whole target biosynthetic pathway to produce the desired product. The emergence of modular co-culture engineering, which divides the pathway between multiple co-culture strains, presents a new perspective to conduct heterologous biosynthesis and improve the bioproduction performance of natural products. This review highlights recent advances in utilizing the modular co-culture engineering approaches to address the challenges of plant and fungal natural product biosynthesis. Potential directions for future research in this promising field are also discussed.

Keywords

Bioproduction improvement Heterologous biosynthesis Modular co-culture engineering Natural product Plants and fungus 

Notes

Acknowledgements

This work is supported by startup research funds provided by Rutgers, The State University of New Jersey. Tingting Chen is a recipient of CSC PhD fellowship.

References

  1. Ahmadi MK, Pfeifer BA (2016) Recent progress in therapeutic natural product biosynthesis using Escherichia coli. Curr Opin Biotechnol 42:7–12.  https://doi.org/10.1016/j.copbio.2016.02.010 CrossRefGoogle Scholar
  2. Ahmadi MK, Fang L, Moscatello N, Pfeifer BA (2016) E coli metabolic engineering for gram scale production of a plant-based anti-inflammatory agent. Metab Eng 38:382–388.  https://doi.org/10.1016/j.ymben.2016.10.001 CrossRefGoogle Scholar
  3. Becker J, Wittmann C (2016) Systems metabolic engineering of Escherichia coli for the heterologous production of high value molecules-a veteran at new shores. Curr Opin Biotechnol 42:178–188.  https://doi.org/10.1016/j.copbio.2016.05.004 CrossRefGoogle Scholar
  4. Camacho-Zaragoza JM, Hernandez-Chavez G, Moreno-Avitia F, Ramirez-Iniguez R, Martinez A, Bolivar F, Gosset G (2016) Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol. Microb Cell Fact 15:163.  https://doi.org/10.1186/s12934-016-0562-z CrossRefGoogle Scholar
  5. Chen Z, Sun X, Li Y, Yan Y, Yuan Q (2017) Metabolic engineering of Escherichia coli for microbial synthesis of monolignols. Metab Eng 39:102–109.  https://doi.org/10.1016/j.ymben.2016.10.021 CrossRefGoogle Scholar
  6. Cragg GM, Newman DJ (2013) Natural products: a continuing source of novel drug leads. Biochem Biophys Acta 1830:3670–3695.  https://doi.org/10.1016/j.bbagen.2013.02.008 CrossRefGoogle Scholar
  7. Ganesan V, Li Z, Wang X, Zhang H (2017) Heterologous biosynthesis of natural product naringenin by co-culture engineering. Synth Syst Biotechnol 2:236–242.  https://doi.org/10.1016/j.synbio.2017.08.003 CrossRefGoogle Scholar
  8. Gupta SS (1994) Prospects and perspectives of natural products in medicine. Indian J Pharmacol 26:1–12Google Scholar
  9. Hoffmeister D, Keller NP (2007) Natural products of filamentous fungi: enzymes, genes, and their regulation. Nat Prod Rep 24:393–416.  https://doi.org/10.1039/b603084j CrossRefGoogle Scholar
  10. Jones JA, Wang X (2017) Use of bacterial co-cultures for the efficient production of chemicals. Curr Opin Biotechnol 53:33–38.  https://doi.org/10.1016/j.copbio.2017.11.012 CrossRefGoogle Scholar
  11. Jones JA et al (2016) Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids. Metab Eng 35:55–63.  https://doi.org/10.1016/j.ymben.2016.01.006 CrossRefGoogle Scholar
  12. Jones JA et al (2017) Complete biosynthesis of anthocyanins using E. coli polycultures. MBio.  https://doi.org/10.1128/mbio.00621-17 Google Scholar
  13. Kovacic P, Somanathan R (2010) Multifaceted approach to resveratrol bioactivity: focus on antioxidant action, cell signaling and safety. Oxid Med Cell Long 3:86–100.  https://doi.org/10.4161/oxim.3.2.3 CrossRefGoogle Scholar
  14. Lee K-H (2004) Current developments in the discovery and design of new drug candidates from plant natural product leads. J Nat Prod 67:273–283.  https://doi.org/10.1021/np030373o CrossRefGoogle Scholar
  15. Li S, Li Y, Smolke CD (2018a) Strategies for microbial synthesis of high-value phytochemicals. Nat Chem 10:395–404.  https://doi.org/10.1038/s41557-018-0013-z CrossRefGoogle Scholar
  16. Li T, Zhou W, Bi H, Zhuang Y, Zhang T, Liu T (2018b) Production of caffeoylmalic acid from glucose in engineered Escherichia coli. Biotechnol Lett 40:1057–1065.  https://doi.org/10.1007/s10529-018-2580-x CrossRefGoogle Scholar
  17. Liu X et al (2018a) Convergent engineering of syntrophic Escherichia coli coculture for efficient production of glycosides. Metab Eng 47:243–253.  https://doi.org/10.1016/j.ymben.2018.03.016 CrossRefGoogle Scholar
  18. Liu Y et al (2018b) Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol. Metab Eng 45:189–199.  https://doi.org/10.1016/j.ymben.2017.12.009 CrossRefGoogle Scholar
  19. Luo Y et al (2015) Engineered biosynthesis of natural products in heterologous hosts. Chem Soc Rev 44:5265–5290.  https://doi.org/10.1039/c5cs00025d CrossRefGoogle Scholar
  20. Marmann A, Aly AH, Lin W, Wang B, Proksch P (2014) Co-cultivation: a powerful emerging tool for enhancing the chemical diversity of microorganisms. Mar Drugs 12:1043–1065.  https://doi.org/10.3390/md12021043 CrossRefGoogle Scholar
  21. Niu FX, He X, Wu YQ, Liu JZ (2018) Enhancing production of pinene in Escherichia coli by using a combination of tolerance, evolution, and modular co-culture engineering. Front Microbiol 9:1623.  https://doi.org/10.3389/fmicb.2018.01623 CrossRefGoogle Scholar
  22. Schueffler A, Anke T (2014) Fungal natural products in research and development. Nat Prod Rep 31:1425–1448.  https://doi.org/10.1039/c4np00060a CrossRefGoogle Scholar
  23. Sgobba E et al (2018) Synthetic Escherichia coli-Corynebacterium glutamicum consortia for l-lysine production from starch and sucrose. Bioresour Technol 260:302–310.  https://doi.org/10.1016/j.biortech.2018.03.113 CrossRefGoogle Scholar
  24. Thuan NH, Chaudhary AK, Van Cuong D, Cuong NX (2018a) Engineering co-culture system for production of apigetrin in Escherichia coli. J Ind Microbiol Biotechnol 45:175–185.  https://doi.org/10.1007/s10295-018-2012-x CrossRefGoogle Scholar
  25. Thuan NH, Trung NT, Cuong NX, Van Cuong D, Van Quyen D, Malla S (2018b) Escherichia coli modular coculture system for resveratrol glucosides production. World J Microbiol Biotechnol 34:75.  https://doi.org/10.1007/s11274-018-2458-z CrossRefGoogle Scholar
  26. Wang J et al (2018) A novel process for cadaverine bio-production using a consortium of two engineered Escherichia coli. Front Microbiol 9:1312.  https://doi.org/10.3389/fmicb.2018.01312 CrossRefGoogle Scholar
  27. Zhang H, Wang X (2016) Modular co-culture engineering, a new approach for metabolic engineering. Metab Eng 37:114–121.  https://doi.org/10.1016/j.ymben.2016.05.007 CrossRefGoogle Scholar
  28. Zhang H, Wang Y, Pfeifer BA (2008) Bacterial hosts for natural product production. Mol Pharm 5:212–225.  https://doi.org/10.1021/mp7001329 CrossRefGoogle Scholar
  29. Zhang H, Boghigian BA, Armando J, Pfeifer BA (2011) Methods and options for the heterologous production of complex natural products. Nat Prod Rep 28:125–151.  https://doi.org/10.1039/c0np00037j CrossRefGoogle Scholar
  30. Zhang H, Pereira B, Li Z, Stephanopoulos G (2015) Engineering Escherichia coli coculture systems for the production of biochemical products. Proc Natl Acad Sci USA 112:8266–8271.  https://doi.org/10.1073/pnas.1506781112 CrossRefGoogle Scholar
  31. Zhang S, Wang S, Zhan J (2016) Engineered biosynthesis of medicinally important plant natural products in microorganisms. Curr Top Med Chem 16:1740–1754CrossRefGoogle Scholar
  32. Zhang W et al (2017) Production of naringenin from d-xylose with co-culture of E. coli and S. cerevisiae. Eng Life Sci 17:1021–1029.  https://doi.org/10.1002/elsc.201700039 CrossRefGoogle Scholar
  33. Zhou K, Qiao K, Edgar S, Stephanopoulos G (2015) Distributing a metabolic pathway among a microbial consortium enhances production of natural products. Nat Biotechnol 33:377.  https://doi.org/10.1038/nbt.3095 CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Chemical and Biochemical EngineeringXiamen UniversityXiamenChina
  2. 2.Department of Chemical and Biochemical EngineeringRutgers, The State University of New JerseyPiscatawayUSA

Personalised recommendations