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Biotechnology Letters

, Volume 40, Issue 9–10, pp 1343–1353 | Cite as

Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability

  • Seyed Ziyae Aldin Samsam Shariat
  • Fatemeh Borzouee
  • Mohammad Reza Mofid
  • Jaleh Varshosaz
Original Research Paper
  • 49 Downloads

Abstract

Objectives

The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS).

Methods

Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using glutaraldehyde as cross-linker. Kinetic properties and stability of free and immobilized LPO were investigated.

Results

LPO was purified 59.13 fold with a specific activity of 5.78 U/mg protein. The successful immobilization of LPO on functionalized GO-NS was confirmed by using dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The overall results showed that the stability of the immobilized LPO was considerably improved compared to free LPO. Apparent Km and Vmax of LPO also indicated that the immobilized enzyme had greater affinity to the substrate than the native enzyme.

Conclusions

Graphene oxide nanosheets are effective means for immobilization of LPO.

Keywords

Lactoperoxidase Immobilization Activity Stability Glutaraldehyde Graphene oxide nanosheets 

Notes

Acknowledgements

The present work was supported by the Grant no. 393740 from Isfahan University of Medical Sciences.

Compliance with ethical standard

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Biochemistry, Bioinformatics Research Center, School of Pharmacy and Pharmaceutical SciencesIsfahan University of Medical SciencesEsfahānIran
  2. 2.Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research CentreIsfahan University of Medical SciencesEsfahānIran

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