TERT enhances the survival rate of human fibroblasts under endoplasmic reticulum, Golgi apparatus, and lysosomal stresses

  • Amer Ali Abd El-Hafeez
  • Toru Hosoi
  • Kanako Nakatsu
  • Mina Thon
  • Akira Shimamoto
  • Hidetoshi Tahara
  • Koichiro Ozawa
Original Research Paper
  • 118 Downloads

Abstract

Objective

The exposure of organelles, such as the endoplasmic reticulum (ER), Golgi apparatus (GA), and lysosomes, to stress activates death mechanisms. Recently, telomerase reverse transcriptase (TERT) has been shown to be involved in cell survival. However, the relationship between TERT and the stress responses is still unclear. Here, we aimed to clarify the possible mechanisms of action through which TERT promotes cell survival by studying its effect on the stresses faced by multiple organelles in human fibroblasts.

Results

We found that TERT enhanced the survival rate of cells under ER stress, regardless of ER stress inducers such as tunicamycin (protein glycosylation inhibitor), thapsigargin (Ca2+-ATPase inhibitor), brefeldin A (protein transport inhibitor), or dithiothreitol (disulfide bond formation inhibitor). We also found that TERT enhanced the survival rate of cells under GA and lysosomal stresses.

Conclusion

Collectively, these results suggest that TERT suppresses cell stress and promotes cell survival via different mechanisms. These findings may offer new insights into the implications of TERT in the treatment of stress-induced conditions such as aging, obesity, and neurodegenerative diseases.

Keywords

Fibroblast Multiple organelle stress Survival rate Telomerase reverse transcriptase 

Notes

Acknowledgements

This work was supported by the JSPS KAKENHI and Takeda Science Foundations.

Supporting information

Supplementary Fig. 1—Overexpression of TERT in the TIG-3-TERT cell line.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical statement

The authors declare that there are no studies conducted with human participants or animals.

Supplementary material

10529_2018_2544_MOESM1_ESM.pptx (113 kb)
Supplementary material 1 (PPTX 112 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pharmacotherapy, Graduate School of Biomedical and Health SciencesHiroshima UniversityHiroshimaJapan
  2. 2.Global Career Design CenterHiroshima UniversityHigashi-Hiroshima CityJapan
  3. 3.Pharmacology and Experimental Oncology Unit, Cancer Biology DepartmentNational Cancer Institute, Cairo UniversityCairoEgypt
  4. 4.Department of Cellular and Molecular Biology, Graduate School of Biomedical and Health SciencesHiroshima UniversityHiroshimaJapan

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