TERT enhances the survival rate of human fibroblasts under endoplasmic reticulum, Golgi apparatus, and lysosomal stresses
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The exposure of organelles, such as the endoplasmic reticulum (ER), Golgi apparatus (GA), and lysosomes, to stress activates death mechanisms. Recently, telomerase reverse transcriptase (TERT) has been shown to be involved in cell survival. However, the relationship between TERT and the stress responses is still unclear. Here, we aimed to clarify the possible mechanisms of action through which TERT promotes cell survival by studying its effect on the stresses faced by multiple organelles in human fibroblasts.
We found that TERT enhanced the survival rate of cells under ER stress, regardless of ER stress inducers such as tunicamycin (protein glycosylation inhibitor), thapsigargin (Ca2+-ATPase inhibitor), brefeldin A (protein transport inhibitor), or dithiothreitol (disulfide bond formation inhibitor). We also found that TERT enhanced the survival rate of cells under GA and lysosomal stresses.
Collectively, these results suggest that TERT suppresses cell stress and promotes cell survival via different mechanisms. These findings may offer new insights into the implications of TERT in the treatment of stress-induced conditions such as aging, obesity, and neurodegenerative diseases.
KeywordsFibroblast Multiple organelle stress Survival rate Telomerase reverse transcriptase
This work was supported by the JSPS KAKENHI and Takeda Science Foundations.
Supplementary Fig. 1—Overexpression of TERT in the TIG-3-TERT cell line.
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Conflict of interest
The authors declare no conflicts of interest.
The authors declare that there are no studies conducted with human participants or animals.
- Brown MK, Naidoo N (2012) The endoplasmic reticulum stress response in aging and age-related diseases. Front Physiol 16:3–263Google Scholar
- Kirkegaard T, Jäättelä M (2009) Lysosomal involvement in cell death and cancer. Biochimica et Biophysica Acta (BBA)-Molecular. Cell Res 1793:746–754Google Scholar
- Machamer CE (2015) The Golgi complex in stress and death. Front Neurosci 6:9–421Google Scholar
- Mattson MP, Zhang P, Fu W (2013) Roles for TERT and Telomerase in Cell Differentiation and Apoptosis. in: Madame Curie Bioscience Database [Internet]. Austin (TX): Landes Bioscience. https://www.ncbi.nlm.nih.gov/books/NBK6129/ 2000–2013
- Milisav I (2011) Cellular stress responses. In: advances in regenerative medicine. InTech, https://doi.org/10.5772/26118, https://www.intechopen.com/books/advances-in-regenerative-medicine/cellular-stress-responses 2011