Biotechnology Letters

, Volume 40, Issue 5, pp 829–836 | Cite as

Flocculation and pentadecane production of a novel filamentous cyanobacterium Limnothrix sp. strain SK1-2-1

  • Takuya Sugawara
  • Mariko Chinzei
  • Setsuko Numano
  • Chifumi Kitazaki
  • Munehiko Asayama
Original Research Paper

Abstract

Objective

A novel filamentous cyanobacterium, a photosynthesizing microorganism, was isolated from a river, and its unique features of flocculation and pentadecane production were characterized.

Results

Microscopic observations and a phylogenetic analysis with 16S rDNA revealed that this strain was a Limnothrix species denoted as the SK1-2-1 strain. Auto cell-flocculation was observed when this strain was exposed to a two-step incubation involving a standing cultivation following a shaking preincubation. Flocculation was enhanced by blue light at a wavelength at 470 nm and irradiation for several hours to 1 day. Moreover, the strain exhibiting exponential cell growth may preferentially accumulate alkanes as pentadecane C15H32 alkane, which may be used as jet fuel, at a range of approximately 1% in the dry cell weight of flocculated cells.

Conclusion

This is the first study on biofuel production using flocculated cells in which the specific manner of production may be regulated by cultivation conditions.

Keywords

Alkane Blue light Cell aggregation Filamentous cyanobacteria Jet fuel Polysaccharide 

Notes

Acknowledgements

This work was partially supported by a propulsive project from Ibaraki University (to MA) and PRESTO (Sakigake project) of the Japan Science and Technology Agency (to MA).

Supporting information

Supplementary Fig. 1—Cyanobacterium SK1-2-1 cells.

Supplementary Fig. 2—Phylogenetic analysis.

Supplementary material

10529_2018_2525_MOESM1_ESM.doc (554 kb)
Supplementary material 1 (DOC 554 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Takuya Sugawara
    • 1
  • Mariko Chinzei
    • 1
  • Setsuko Numano
    • 1
  • Chifumi Kitazaki
    • 1
  • Munehiko Asayama
    • 1
    • 2
    • 3
  1. 1.Laboratory of Molecular Genetics, College of AgricultureIbaraki UniversityIbarakiJapan
  2. 2.Japan Science and Technology Agency (JST)Precursory Research for Embryonic Science and Technology (PRESTO)SaitamaJapan
  3. 3.United Graduate School of Agricultural ScienceTokyo University of Agriculture and TechnologyTokyoJapan

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