Biotechnology Letters

, Volume 41, Issue 2, pp 221–230 | Cite as

Progress in antibiotic susceptibility tests: a comparative review with special emphasis on microfluidic methods

  • Zeeshan A. Khan
  • Mohd F. Siddiqui
  • Seungkyung ParkEmail author


Antibiotic susceptibility test (AST) is an umbrella term for techniques to determine the susceptibility of bacteria to antibiotics. The antibiotic-resistant bacteria are a major threat to public health and a directed therapy based on accurate AST results is paramount in resistance control. Here we have briefly covered the progress of conventional, molecular, and automated AST tools and their limitations. Various aspects of microfluidic AST such as optical, electrochemical, colorimetric, and mechanical methods have been critically reviewed. We also address the future requirements of the microfluidic devices for AST. Cumulatively, we have outlined the overview of AST that can help to expand and improve the existing techniques and emphasize that microfluidics could be the future of AST and introduction of microtechnologies in AST will be extremely advantageous, especially for point-of-care testing.


Antibiotic susceptibility test Resistant bacteria Microfluidic methods Infectious disease Automated system 


Authors’ contributions

ZK, MS, and SP wrote and edited the manuscript. All the authors read and approved the final manuscript.


This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2015R1C1A1A01054762).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Media B.V. 2018

Authors and Affiliations

  1. 1.School of Mechanical EngineeringKorea University of Technology and EducationCheonanSouth Korea

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