Oligosaccharides as co-encapsulating agents: effect on oral Lactobacillus fermentum survival in a simulated gastrointestinal tract
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Four kinds of oligosaccharides were used as co-encapsulating agents to test the effect of extrusion-based microencapsulation on protection of Lactobacillus fermentum L7 against exposure to simulated gastric and intestinal juices as well as long-term refrigeration storage at 4 °C.
The combination of alginate with galacto-oligosaccharides, isomalto-oligosaccharides, fructo-oligosaccharides, and xylo-oligosaccharides, or alginate alone exhibited good properties of the beads. The diameters of the cell beads co-encapsulated with oligosaccharides and encapsulated with alginate alone were similar, in the range of 2.34–2.51 mm. However, the encapsulation yield of L. fermentum cells co-encapsulated with oligosaccharides, which was in the range of 79.52–89.75%, was significantly higher than that of cells encapsulated with alginate alone. The capsules were stable in gastric conditions and can disintegrated when exposed to intestinal conditions. Additionally, the viability of microencapsulated cells after exposure to the simulated gastric and intestinal juices as well as long-term refrigeration storage was better than that of free cells, and the viability of cells co-encapsulated with oligosaccharides was better than that of cells encapsulated with alginate alone. Furthermore, fructo-oligosaccharides used as co-encapsulating agent showed the best performance.
Microencapsulating L. fermentum with oligosaccharides protected cells well at a low temperature and offered effective gastrointestinal delivery of probiotics, and thus has the potential to maintain bacterial survival in probiotic products and will provide the research basis for design of effective probiotic-prebiotic combinations to maximize host benefit.
KeywordsLactobacillus fermentum Microencapsulation Oligosaccharides Simulated gastrointestinal digestion Sodium alginate
This work was supported by the National Natural Science Foundation of China (NO. 31760446), and the High Level Talent Startup Project of Shihezi University (NO. CZX201432R).
- Abbaszadeh S, Gandomi H, Misaghi A, Bokaei S, Noori N (2014) The effect of alginate and chitosan concentrations on some properties of chitosan-coated alginate beads and survivability of encapsulated Lactobacillus rhamnosus in simulated gastrointestinal conditions and during heat processing. J Sci Food Agr 94:2210–2216. https://doi.org/10.1002/jsfa.6541 CrossRefGoogle Scholar
- Chavarri M, Maranon I, Ares R, Ibanez FC, Marzo F, Villaran MD (2010) Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int J Food Microbiol 142:185–189. https://doi.org/10.1016/j.ijfoodmicro.2010.06.022 CrossRefGoogle Scholar
- Fang X, Duan RS, Wang FS (2014) Effects of oligosaccharides on the bioactivity of Lactobacillus. Pharma Biotechnol 21(4):338–442. https://doi.org/10.19526/j.cnki.1005-8915.2014.04.013 Google Scholar
- Gebara C, Chaves KS, Ribeiro MCE, Souza FN, Grosso CRF, Gigante ML (2013) Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles during exposure to simulated gastrointestinal conditions. Food Res Int 51(2):872–878. https://doi.org/10.1016/j.foodres.2013.02.008 CrossRefGoogle Scholar
- Mirzaei H, Pourjafar H, Homayouni A (2012) Effect of calcium alginate and resistant starch microencapsulation on the survival rate of Lactobacillus acidophilus La5 and sensory properties in Iranian white brined cheese. Food Chem 132:1966–1970. https://doi.org/10.1016/j.foodchem.2011.12.033 CrossRefGoogle Scholar
- Niu CH et al (2015) Effect of Prebiotic Oligosaccharides on Promoting Growth of Lactobacillus fermentum CH4. J Jilin Agric Sci 40(2):105–109. https://doi.org/10.16423/j.cnki.1003-8701.2015.02.027 Google Scholar
- Sandoval-Castilla O, Lobato-Calleros C, Garcia-Galindo HS, Alvarez-Ramirez J, Vernon-Carter EJ (2010) Textural properties of alginate-pectin beads and survivability of entrapped Lb. casei in simulated gastrointestinal conditions and in yoghurt. Food Res Int 43(1):111–117. https://doi.org/10.1016/j.foodres.2009.09.010 CrossRefGoogle Scholar
- Zhong JJ et al (2007) Pharmaceutical significance of sodium alginate. Chin J New Drugs 16:591–594Google Scholar