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Morphological Characterization and Gene Expression Patterns for Melanin Pigmentation in Rex Rabbit

  • Shuaishuai Hu
  • Pin Zhai
  • Yang Chen
  • Bohao Zhao
  • Naisu Yang
  • Manman Wang
  • Yeyi Xiao
  • Guolian Bao
  • Xinsheng WuEmail author
Original Article
  • 22 Downloads

Abstract

Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair follicles and melanin content in multi-colored Rex rabbit skins were observed by Hematoxylin and Eosin (H&E) staining and melanin staining, respectively. The melanin granules were primarily found in the epidermis and hair follicle roots. The melanin content of skin was measured by extracting melanin from skin tissue. The results demonstrated that the melanin content was the highest in the skin of black Rex rabbit. Additionally, we measured the mRNA and protein expression levels of melanin-related key genes (MITF and TYR) in the skin of different hair color by quantitative real-time PCR and Wes assay, respectively. The results revealed that the mRNA expression levels in the skin of black Rex rabbit was highly expressed when as compared with other Rex rabbit skin (P < 0.01), and they were the lowest in the skin of white Rex rabbit. Finally, correlation analysis was conducted between melanin content and the expression levels of mRNA and protein. The results indicated a significant correlation between melanin content and the mRNA expression of MITF (P < 0.05), but it was not correlated with the mRNA expression of TYR (P > 0.05). In summary, melanin deposition has important economic value, and the coat color of fur-bearing animals is partly determined by the melanin-related genes.

Keywords

Rex rabbit Skin Melanin Gene expression 

Notes

Funding

This work was supported by the Modern Agricultural Industrial System Special Funding (CARS-43-A-1), the National Natural Science Foundation of China (3170130120), and the Science and Technology Major Project of New Variety Breeding (Livestock and Poultry) of Zhejiang Province, China (2016C02054-10).

Compliance with Ethical Standards

Conflict of interest

ShuaiShuai Hu, Yang Chen, BoHao Zhao, NaiSu Yang, ManMan Wang, Yeyi Xiao, Guolian Bao, and Xinsheng Wu declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shuaishuai Hu
    • 1
  • Pin Zhai
    • 2
  • Yang Chen
    • 1
  • Bohao Zhao
    • 1
  • Naisu Yang
    • 1
  • Manman Wang
    • 1
  • Yeyi Xiao
    • 1
  • Guolian Bao
    • 3
  • Xinsheng Wu
    • 1
    Email author
  1. 1.College of Animal Science and TechnologyYangzhou UniversityYangzhouChina
  2. 2.Animal Husbandry and Veterinary Research InstituteJiangsu Academy of Agricultural SciencesNanjing, JiangsuChina
  3. 3.Animal Husbandry and Veterinary Research InstituteZhejiang Academy of Agricultural SciencesHangzhouChina

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