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Comparative Analysis of Expression Profiles of Reg Signaling Pathways-Related Genes Between AHF and HCC

  • Gaiping WangEmail author
  • Liya Cheng
  • Meng Chen
  • Congcong Zhao
  • Mingxin Gao
  • Tiantian Huang
  • Peipei Chu
  • Cunshuan Xu
Original Article

Abstract

Regenerating islet-derived protein (Reg) could participate in the occurrence of diabetes mellitus, inflammation, tumors, and other diseased or damaged tissues. However, the correlation of Reg with acute hepatic failure (AHF) and hepatocellular carcinoma (HCC) is poorly defined. To reveal the expression profiles of Reg family and their possible regulatory roles in AHF and HCC, rat models of HCC and AHF were separately established, and Rat Genome 230 2.0 was used to detect expression profiles of Reg-mediated signaling pathways-associated genes from liver tissues in AHF and HCC. The results showed that a total of 79 genes were significantly changed. Among these genes, 67 genes were the AHF-specific genes, 45 genes were the HCC-specific genes, and 33 genes were the common genes. Then, K-means clustering classified these genes into 4 clusters based on the gene expression similarity, and DAVID analysis showed that the above altered genes were mainly associated with stress response, inflammatory response, and cell cycle regulation. Thereafter, IPA software was used to analyze potential effects of these genes, and the predicted results suggested that the Reg-mediated JAK/STAT, NF-κB, MAPK (ERK1/2, P38 and JNK), PLC, and PI3K/AKT signaling pathways may account for the activated inflammation and cell proliferation, and the attenuated apoptosis and cell death during the occurrence of AHF and HCC.

Keywords

Regenerating islet-derived protein Acute hepatic failure Hepatocellular carcinoma Gene expression profile Physiological activities 

Notes

Acknowledgements

Great thanks should be given to Liya Cheng for preparing experimental materials, Mingxin Gao, Tiantian Huang and Peipei Chu for data analysis, and Meng Chen and Congcong Zhao for revising this manuscript. This work was financially supported by the Henan Scientific and Technological Research Project (182102310244), the Key Scientific Research Projects of Henan Higher Education (18A180019), the Graduate Scientific Research Innovation Project of Henan Normal University (YL201811), and Henan Normal University’s Crossing Research Project (20170008) with Xinxiang Giant Industry and Trade Co, Ltd.

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Gaiping Wang
    • 1
    • 2
    Email author
  • Liya Cheng
    • 1
    • 2
  • Meng Chen
    • 1
    • 2
  • Congcong Zhao
    • 1
    • 2
  • Mingxin Gao
    • 1
  • Tiantian Huang
    • 1
    • 2
  • Peipei Chu
    • 1
    • 2
  • Cunshuan Xu
    • 1
    • 2
  1. 1.College of Life ScienceHenan Normal UniversityXinxiangChina
  2. 2.State Key Laboratory Cultivation Base for Cell Differentiation RegulationHenan Normal UniversityXinxiangChina

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