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Identification and Characterization of a Novel Strigolactone-Insensitive Mutant, Dwarfism with High Tillering Ability 34 (dhta-34) in Rice (Oryza sativa L.)

  • Rong Liang
  • Ran Qin
  • Chengcong Yang
  • Dongdong Zeng
  • Xiaoli Jin
  • Chunhai Shi
Original Article
  • 88 Downloads

Abstract

Rice tillering ability and plant height are two of the important traits determining the grain yield. A novel rice (Oryza sativa L.) mutant dhta-34 from an Indica cultivar Zhenong 34 treated by ethyl methy1 sulfonate (EMS) was investigated in this study. The dhta-34 mutant significantly revealed thrifty tillers with reduced plant height, smaller panicles and lighter grains. It also exhibited late-maturing (19.80 days later than the wild type) and withered leaf tip during the mature stage. The length of each internode was reduced compared to the wild type, belonging to the dn type (each internode of the plant stem decreased in the same ratio). The longitudinal section of dhta-34 internodes showed that the length of cells was reduced leading to the dwarfism of the mutant. The F2 population derived from a cross between dhta-34 and an Japonica cultivar Zhenongda 104 were used for gene mapping by using the map-based cloning strategy. The gene DHTA-34 was fine mapped in 183.8kb region flanked by markers 3R-7 and 3R-10. The cloning and sequencing of the target region from the mutant revealed that there was a substitution of G to A in the second exon of LOC_Os03g10620, which resulted in an amino acid substitution arginine to histidine. DHTA-34 encoded a protein of the α/β-fold hydrolase superfamily, which could suppress the tillering ability of rice. DHTA-34 was a strong loss-of-function allele of the Arabidopsis thaliana D14 gene, which was involved in part of strigolactones (SLs) perception and signaling. Moreover, the relative expression of DHTA-34 gene in leaf was higher than that in bud, internode, root or sheath. This study revealed that DHTA-34 played an important role in inhabiting tiller development in rice and further identifying the function of D14.

Keywords

DHTA-34 (dwarfism with high tillering ability 34Strigolactones (SLs) Fine mapping Dwarfism and high tillering ability Rice 

Abbreviations

dhta-34

Dwarfism with high tillering ability 34

EMS

Ethyl methyl sulfonate

FAA

Formalin-acetic acid-alcohol

SLs

Strigolactones

Notes

Acknowledgments

This work was supported by the Science and Technology Office of Zhejiang Province (2016C32085 and 2012C12901-2), Open Foundation of Key Laboratory for Crop Germplasm of Zhejiang Province and the Program for Innovative Research Team in University (IRT1185).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest to this work and have no financial and personal relationships with other people or organizations that can inappropriately influence their work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled.

Supplementary material

10528_2018_9896_MOESM1_ESM.docx (35 kb)
Supplementary file1 (DOCX 35 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rong Liang
    • 1
  • Ran Qin
    • 1
  • Chengcong Yang
    • 1
  • Dongdong Zeng
    • 1
  • Xiaoli Jin
    • 1
  • Chunhai Shi
    • 1
  1. 1.Department of AgronomyZhejiang UniversityHangzhouChina

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