Biochemical Genetics

, Volume 57, Issue 4, pp 477–486 | Cite as

Omega-3 PUFA Alters the Expression Level but Not the Methylation Pattern of the WIF1 Gene Promoter in a Pancreatic Cancer Cell Line (MIA PaCa-2)

  • Babak Rahmani
  • Dariush Hamedi Asl
  • Taghi Naserpour Farivar
  • Mehdi Azad
  • Mehdi SahmaniEmail author
  • Nematollah GheibiEmail author
Original Article


Pancreatic cancer is the fourth leading cause of death in both males and females, with a 5-year relative survival rate of 8%. The Wnt signaling pathway has a significant role in the pathogenesis of many tumors, including those of pancreatic cancer. Hypermethylation of the Wnt inhibitory Factor-1 (WIF1) gene promoter have been detected in different types of cancer. In contrast, the anticancer effects of long-chain omega-3 PUFA (ALA) have been reported. Regarding its anticancer effects, in this study, we investigated the effects of various concentrations of omega-3 PUFA on expression level and promoter methylation of the WIF1 gene in MIA PaCa-2 cells in 24, 48, and 72 h after treatment. MIA PaCa-2 cells were treated with different concentrations of omega-3 PUFA (25, 50, 100, 250, 500, and 1000 μM). Cell viability assay was carried out followed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and methylation-specific PCR (MSP). This investigation suggested that dietary consumption of omega-3 PUFAs (250–1000 μM) has a significant effect on the proliferation and WIF1 gene expression of the MIA PaCa-2 cancer cell line but no effect on the promoter methylation of this gene. Changes in promoter methylation were not observed in any of the treatments.


Omega-3 PUFA Pancreatic cancer MIA PaCa-2 Methylation MSP 



This work was supported by a Grant from the Deputy for Research and Technology, Qazvin University of Medical Sciences, Qazvin, Iran.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Molecular Medicine, Faculty of Medical SciencesQazvin University of Medical SciencesQazvinIran
  2. 2.Cellular and Molecular Research CentreQazvin University of Medical SciencesQazvinIran
  3. 3.Department of Medical Laboratory Sciences, Faculty of Allied MedicineQazvin University of Medical SciencesQazvinIran
  4. 4.Department of Clinical Biochemistry and Genetic, Faculty of MedicineQazvin University of Medical SciencesQazvinIran

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