Morphometrics and Mitochondrial DNA Genes Analysis Suggest a New Species of Penaeus (Crustacea: Penaeidae) from the Persian Gulf
- 42 Downloads
There are two morphotypes of Penaeus semisulcatus described hitherto in the Persian Gulf, namely the banded and non-banded antennae morphotypes. In this study, we used morphometric measurements and two mitochondrial genes (16S rRNA and cytochrome oxidase subunit I—COI) to assess relationships between the two morphotypes of P. semisulcatus. Out of 25 morphological characters examined, 10 characters were found significantly different between the two morphotypes when tested against separate sexes or both sexes combined. Results from the 16S rRNA and COI sequence analysis of two morphotypes of P. semisulcatus morphotype showed up to 6% and 17% sequence divergence, respectively. The 16S rDNA and COI sequences of the non-banding morphotype were not only very different to those of the banding morphotype but was also very different to all other Penaeus species (i.e., P. monodon, P. merguiensis, and P. indicus) included in the study. Both parsimony and Neighbor-Joining trees based on 16S rDNA and COI sequences provide similar tree topology that clearly separated the two morphotypes into two distinct groups. Based on these findings, we propose the two morphotypes of P. semisulcatus to be relegated as two sympatric species.
KeywordsPenaeus semisulcatus mtDNA Morphometric Persian Gulf 16S rRNA COI
The authors wish to thank the Iranian Fisheries Sciences Research Institute, for helpful assistance. We thank the head of Persian Gulf and Oman Sea Ecological Research Institute, This research was funded by Postgraduate Research Grant Scheme from Universiti Sains Malaysia.
Compliance with Ethical Standards
Conflict of interest
The authors do not have any conflict of interest.
- Alam MMM, de Cross MDST, Pálsson S (2016) Mitochondrial DNA variation reveals distinct lineages in Penaeus semisulcatus (Decapoda, Penaeidae) from the Indo-West Pacific Ocean. Mar Ecol 38:1–11Google Scholar
- De Haan (1844) Penaeus semisulcatus, in Von Siebold, Fauna Japonica, Crustacea (6/7): Pl. 46. In: Holthuis LB (ed), FAO catalogue. Shrimps and prawns of the world. An annotated catalogue of species of interest to fisheries, vol 1, no 125. Rijksmuseum of Natural History Leiden, The NetherlandsGoogle Scholar
- FAO (2016) The state of world fisheries and aquaculture. FAO, RomeGoogle Scholar
- Grey D, Dall W, Baker A (1983). A guide to the Australian Penaeid Prawns. Northern Territory Government Printing Office, The Department of Primary Production of the Northern Territory, Darwin, AustraliaGoogle Scholar
- Gusmão J, Lazoski C, Solé-Cava AM (2000) A new species of Penaeus (Crustacea: Penaeidae) revealed by allozyme and cytochrome oxidase I analyses. Mar Biol 37:435–446Google Scholar
- Hayashi KI (1992) Dendobranchiata Crustaceans from Japanese Waters. Seibutsu Kenkyusha, Tokyo, p 300Google Scholar
- Holthuis LB (1980) Shrimp and prawns of the word. An annotated catalogue of species of interest to fisheries. FAO Species Catalog, RomeGoogle Scholar
- Meyer A (1994) DNA technology and phylogeny of fish. In: Beaumont AR (ed) Genetics and the evolution of Aquatic Organisms Beaumont. Chapman and Hall, London, pp 219–249Google Scholar
- Palumbi SR, Benzie JAH (1991) Large mitochondrial DNA differences between morphologically similar Penaeid shrimp. Mol Mar Biol Biotech 1:27–34Google Scholar
- Paugy D, Leveque C (1999) Taxinomie et systématique. In: Lévêque C, Paugy D (eds) Les poissons des eaux continentales africaines Diversité, écologie et utilisation par l’homme. Edition IRD, Paris, pp 97–119Google Scholar
- Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Schubart CD, Neigel JE, Felder DL (2000) Use of the mitochondrial 16S rRNA gene for phylogenetic and population studies of Crustacea. Crustac Issues 12:817–830Google Scholar
- Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, SunderlandGoogle Scholar
- Tzeng TD, Yeh SY (1999) Analysis of the morphometric characters of the kuruma shrimp (Penaeus japonicus) in the East China Sea and the Taiwan Strait. Taipei J Fish Soc Taiwan 26:203–212Google Scholar
- Yu HP, Chan TY (1986) The illustrated penaeoid prawns of Taiwan. Southern Materials Center Inc, TaipeiGoogle Scholar
- Zar JH (1996) Biostatistical analysis. Prentice Hall, Upper Saddle RiverGoogle Scholar