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Association Analysis of IGF2BP2, KCNJ11, and CDKAL1 Polymorphisms with Type 2 Diabetes Mellitus in a Moroccan Population: A Case–Control Study and Meta-analysis

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Abstract

Associations with type 2 diabetes mellitus have been identified for variants CDKAL1 rs7756992, KCNJ11 rs5219, and IGF2BP2 rs4402960 in different populations. In a case–control study of 250 unrelated Moroccan diabetic patients and 250 healthy controls, we used TaqMan allelic discrimination assays to genotype the three SNPs and meta-analysis to investigate the association between the polymorphisms and diabetes in Arab populations. The results showed a significant diabetes association only with the variant rs4402960 of the IGF2BP2 gene under additive 2 (GG vs. TT; p = 0.009) and recessive (TT vs. GG+GT; p = 0.003) models. Meta-analysis indicated significant association between the IGF2BP2 rs4402960 and CDKAL1 rs7756992 polymorphisms and increased risk of diabetes in Arab populations. According to our results, the case–control study and meta-analysis revealed a significant association between the IGF2BP2 rs4402960 variant and type 2 diabetes in Moroccan and Arab populations.

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Acknowledgments

This work was supported by NEPAD/NABNet New Partnership for Africa’s Development, North Africa Biosciences Network, and Joint WHO/EMRO-COMSTECH RAB&GH grants. The authors gratefully acknowledge the patients with diabetes and their families for their participation in this study.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire Humaine, Département de Recherche Scientifique, Institut Pasteur du Maroc, Place Louis Pasteur, 20100, Casablanca, Morocco

    Houda Benrahma, Hicham Charoute, Redouane Boulouiz, Hassan Rouba & Abdelhamid Barakat

  2. Laboratoire Agroalimentaire et Santé, Univ Hassan 1, 26000, Settat, Morocco

    Hicham Charoute & Malika Fakiri

  3. Laboratory of Biomedical Genomics and Oncogenetics LR11IPT05, Institut Pasteur de Tunis, 1002, Tunis, Tunisia

    Khaled Lasram, Rym Kefi-Ben Atig & Sonia Abdelhak

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  1. Houda Benrahma
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  2. Hicham Charoute
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  3. Khaled Lasram
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  4. Redouane Boulouiz
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  6. Malika Fakiri
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  7. Hassan Rouba
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  8. Sonia Abdelhak
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  9. Abdelhamid Barakat
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Correspondence to Abdelhamid Barakat.

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Houda Benrahma and Hicham Charoute contributed equally to this work.

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Benrahma, H., Charoute, H., Lasram, K. et al. Association Analysis of IGF2BP2, KCNJ11, and CDKAL1 Polymorphisms with Type 2 Diabetes Mellitus in a Moroccan Population: A Case–Control Study and Meta-analysis. Biochem Genet 52, 430–442 (2014). https://doi.org/10.1007/s10528-014-9658-5

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