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Detection of Major Lymphocyte Populations by Image Cytometry

  • V. E. Kurochkin
  • I. V. Chubinskiy-Nadezhdin
  • E. K. Vasil’evaEmail author
  • Yu. V. Kulikov
  • B. V. Bardin
  • A. I. Martynov
Article

A detector of cell subpopulations developed at the Institute of Analytical Instrumentation of the Russian Academy of Sciences was used for immunophenotyping lymphocytes from healthy donors and standardized blood samples with normal and low-level cell populations. It has been shown that it is possible (provided that the modern criteria for the accuracy of the analysis are fulfilled) to determine the relative content of the main populations of lymphocytes, including in standard samples with reduced content of T helper cells that mimic the blood of patients with HIV infection.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • V. E. Kurochkin
    • 1
  • I. V. Chubinskiy-Nadezhdin
    • 1
  • E. K. Vasil’eva
    • 1
    Email author
  • Yu. V. Kulikov
    • 1
  • B. V. Bardin
    • 1
  • A. I. Martynov
    • 2
  1. 1.Institute of Analytical Instrumentation, Russian Academy of SciencesSt. PetersburgRussia
  2. 2.NRC Institute of Immunology, Federal Medical_Biological AgencyMoscowRussia

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