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Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)

  • Wenxiu Wang
  • Lifeng Zhou
  • Guangping Dong
  • Fengmao ChenEmail author


In China, the fall webworm, Hyphantria cunea, causes enormous losses. The potential of entomopathogenic fungi (EPF) to control pest insects has been demonstrated in the laboratory and the field. In this study, we isolated and identified EPF from the surfaces of H. cunea larvae cadavers, and we investigated their efficacy against fall webworm larvae under bioassay conditions in the laboratory. Isaria javanica BE01 conidial suspensions with concentrations between 1 × 104 and 1 × 108 conidia ml−1 were shown to have marked effects on the larval mortality (the 3rd, 4th, and 5th instar larvae) over a 15 days period. However, the effect of the instar on the mortality was not always significant, except during the late stages (10–14 days) at low concentrations (1 × 104–1 × 106 conidia ml−1) or the early stages (1–5 days) at high concentrations (1 × 107–1 × 108 conidia ml−1). Otherwise, the mortality of the 3rd and 4th instar larvae exceeded 85% at 1 × 108 conidia ml−1. The present study suggests that I. javanica BE01 is a potential biological control agent of H. cunea.


Isaria javanica Fall webworm Identification Biological control Larval instar Conidial suspensions concentration 



This study was supported by grants from the National Key Research and Development Program of China (2017YFD0600104).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies on humans or animals performed by any of the authors.

Supplementary material

10526_2019_9982_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)


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Copyright information

© International Organization for Biological Control (IOBC) 2019

Authors and Affiliations

  1. 1.Collaborative Innovation Center of Sustainable Forestry in Southern China, College of ForestryNanjing Forestry UniversityNanjingChina
  2. 2.Forestry Academe of Anhui ProvinceHefeiChina

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