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Host testing of Eadya daenerys, a potential biological control agent for the invasive chrysomelid pest Paropsis charybdis, predicts host specificity to eucalypt-leaf feeding Paropsina

  • T. M. WithersEmail author
  • C. L. Todoroki
  • G. R. Allen
  • A. R. Pugh
  • B. A. Gresham


Host range testing of a larval endoparasitoid Eadya daenerys Ridenbaugh (Hymenoptera: Braconidae) was conducted. Eadya daenerys is most commonly associated with Paropsisterna agricola (Chapuis) in Australia, but is proposed as a biological control agent for Paropsis charybdis Stål (Coleoptera: Chrysomelidae) in New Zealand. Nine species of non-target beetles with spring-active, external leaf-feeding larvae, were host tested. Development to emergence only occurred within eucalypt-feeding Paropsina pests: the target P. charybdis and another pest Trachymela sloanei (Blackburn). Unsuccessful internal parasitism occurred in four less closely related non-target Chrysomelinae. Considering the different feeding niches occupied by these beetles, we hypothesize that Eadya daenerys is unlikely to cause direct non-target impacts beyond pest Paropsina species in the New Zealand environment.


Risk assessment Endemic beetle Weed biological control agents Eucalyptus pest 



Many thanks to other Scion staff including Andrew Dunningham, Justin Nairn, Matt Scott, Pam Taylor, Carl Wardhaugh, and University of Tasmania, Institute of Agriculture staff members Vin Patel and Steve Quarrell. Dean Satchell, New Zealand Farm Forestry Association has also been an invaluable contributor to this research. Students have also been highly valued for their assistance with this project, including Stephanie Kirk, Mike Davy, Sean Gatenby, Katherine Moors, Elise Peters and Amy Yasutake-Watson, in New Zealand, and Ray Ali, Hui Law, Meng Lim, Allanna Russell, and Rebekah Smart, in Australia. Thanks to landowners, including Forestry Tasmania, iFarm, PF Olsen, Oji Fibre Solutions (NZ) Ltd, the NZ Defence Force, the NZ Department of Conservation (DOC) and The Westervelt Company NZ Ltd for access to field sites for collecting. Thanks to Landcare Research scientists Ronny Groenteman, Hugh Gourlay, Chris Winks, Paul Peterson and Richard Leschen and AgResearch scientist Mike Cripps for their invaluable assistance with non-target beetles. Allocharis nr tarsalis were collected under DOC research authority 54216RES. Funding was from the New Zealand Ministry of Primary Industries Sustainable Farming Fund contracts 12-039 and 407964, and New Zealand Ministry of Business Innovation and Employment Strategic Science Investment Funding contract to Scion. Industry funding co-partners Southwood Exports Limited and Oji Fibre Solutions NZ Ltd, are gratefully acknowledged, as are the Speciality Wood Products Partnership, and the New Zealand Farm Forestry Association.


  1. Abram PK, Brodeur J, Urbaneja A, Tena A (2019) Nonreproductive effects of insect parasitoids on their hosts. Annu Rev Entomol 64:259–276CrossRefGoogle Scholar
  2. Bain J, Kay MK (1989) Paropsis charybdis Stål, eucalyptus tortoise beetle (Coleoptera: Chrysomelidae). In: Cameron PJ, Hill RL, Bain J, Thomas WP (eds) A review of biological control of invertebrate pests and weeds in New Zealand 1874–1987, Technical Communication No. 10. CAB International and DSIR, Oxon, UK, pp 281–287Google Scholar
  3. Barratt B, Moeed A, Malone L (2006) Biosafety assessment protocols for new organisms in New Zealand: can they apply internationally to emerging technologies? Biol Control 26(4):339–358Google Scholar
  4. Brodeur J, Geervliet JBF, Vet LEM (1998) Effects of Pieris host species on life history parameters in a solitary specialist and gregarious generalist parasitoid (Cotesia species). Ent Exp et Appl 86(2):145–152CrossRefGoogle Scholar
  5. Cripps MG, Gassmann A, Fowler SV, Bourdôt GW, McClay AS, Edwards GR (2011) Classical biological control of Cirsium arvense: lessons from the past. Biol Control 57(3):165–174CrossRefGoogle Scholar
  6. De Little DW (1989) Paropsine chrysomelid attack on plantations of Eucalyptus nitens in Tasmania. N Z J For Sci 19(2–3):223–227Google Scholar
  7. de Mendiburu F (2016) agricolae: statistical procedures for agricultural research. R package version 1.2-4Google Scholar
  8. Fowler S, Barretto R, Dodd S, Macedo DM, Paynter Q, Pedrosa-Macedo J, Pereira O, Peterson P, Smith L, Waipara N, Winks C, Forrester G (2013) Tradescantia fluminensis, an exotic weed affecting native forest regeneration in New Zealand: ecological surveys, safety tests and releases of four biocontrol agents from Brazil. Biol Control 64(3):323–329CrossRefGoogle Scholar
  9. Ge D, Gomez-Zurita J, Chesters D, Yang X-K, Vogler AP (2012) Suprageneric systematics of flea beetles (Chrysomelidae: Alticinae) inferred from multilocus sequence data. Mol Phylogen Evol 62:793–805CrossRefGoogle Scholar
  10. Groenteman R (2013) Prospects for the biological control of tutsan (Hypericum androsaemum L.) in New Zealand. Proceedings of the XIII international symposium of biological control of weeds, pp 128–137.
  11. Haye T, Goulet H, Mason PG, Kuhlmann U (2005) Does fundamental host range match ecological host range? a retrospective case study of a Lygus plant bug parasitoid. Biol Control 35:55–67CrossRefGoogle Scholar
  12. Hoddle MS (2004) Chapter 4. Analysis of fauna in the receiving area for the purpose of identifying native species that exotic natural enemies may potentially attack. In: van Driesche RG, Reardon R (eds) Assessing host ranges for parasitoids and predators used for classical biological control: a guide to best practice, forest health technology enterprise team, report FHTET-2004-03, September 2004. West Virginia, USDA Forest Service, Morgantown, pp 24–39Google Scholar
  13. Jurado-Rivera JA (2014) Filogenia molecular, sistemática y evolución delos Chrysomelinae australianos (Coleoptera, Chrysomelidae). University of the Balearic Islands, Spain, Ph.D.Google Scholar
  14. Jurado-Rivera JA, Vogler AP, Reid CAM, Petitpierre E, Gómez-Zurita J (2009) DNA barcoding insect–host plant associations. Proc R Soc Biol Sci Ser B 276:639–648CrossRefGoogle Scholar
  15. Keller MA (1999) Understanding host selection behaviour: the key to more effective host specificity testing. In: Withers TM, Barton Browne L, Stanley J (eds) Host specificity testing in Australasia: towards improved assays for biological control, vol 1. Scientific Publishing. Department of Natural Resources, Brisbane, pp 84–92Google Scholar
  16. Kuhlmann U, Schaffner U, Mason PG (2006) Selection of non-target species for host specificity testing. In: Bigler F, Babendreier D, Kuhlmann U (eds) Environmental impact of invertebrates for biological control of arthropods. CABI International, Wallingford, pp 15–37CrossRefGoogle Scholar
  17. Leschen RAB (2004) Reid CAM (2004) New Zealand Chrysomelidae. Chrysomela 43:7–8Google Scholar
  18. Mansfield S, Murray TJ, Withers TM (2011) Will the accidental introduction of Neopolycystus insectifurax improve biological control of the eucalyptus tortoise beetle, Paropsis charybdis, in New Zealand? Biol Control 56:30–35CrossRefGoogle Scholar
  19. Memmott J, Godfray HCJ, Gauld ID (1994) The structure of a tropical host-parasitoid community. J Anim Ecol 63(3):521–540CrossRefGoogle Scholar
  20. Millar JG, Paine TD, Hoddle MS (1999) Biological control of a newly introduced pest, the eucalyptus tortoise beetle, Trachymela sloanei. Slosson Rep 2000:1–7Google Scholar
  21. Murray TJ, Withers TM (2011) Spread of Dicranosterna semipunctata (Col.: Chrysomelidae) in New Zealand and potential for control by intentionally introduced and invasive parasitoids. Biol Control 59:234–238CrossRefGoogle Scholar
  22. Paynter Q, Forgie S, Winks C, Peterson P, Ward D, Nicholson L, Zoelen R (2010) Predicting parasitoid accumulation on biological control agents of weeds. J Appl Ecol 47:575–582CrossRefGoogle Scholar
  23. Peixoto L, Allen GR, Ridenbaugh RD, Quarrell SR, Withers TM, Sharanowski BJ (2018) When taxonomy and biological control researchers unite: species delimitation of Eadya parasitoids (Braconidae) and consequences for classical biological control of invasive paropsine pests of Eucalyptus. PLoS ONE 13(8):e0201276CrossRefGoogle Scholar
  24. R Core Team (2019) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Australia.
  25. Reid CAM (1995) A cladistic analysis of subfamilial relationships in the Chrysomelidae sensu lato (Chrysomeloidea). In: Pakaluk J, Slipinski S (eds) Biology, phylogeny and classification of Coleoptera: papers celebrating the 80th birthday of Roy A. Crowson. Muzeum i Instytut Zoologii PAN, Warszawa, pp 559–631Google Scholar
  26. Reid CAM (2006) A taxonomic revision of the Australian Chrysomelinae, with a key to the genera (Coleoptera: Chrysomelidae). Zootaxa 1292:1–119CrossRefGoogle Scholar
  27. Rice AD (2005) The parasitoid guild of larvae of Paropsisterna agricola Chapuis (Coleoptera: Chrysomelidae) in Tasmania, with notes on biology and a description of a new genus and species of tachinid fly. Aust J Entomol 44:400–408CrossRefGoogle Scholar
  28. Rice AD, Allen GR (2009) Temperature and developmental interactions in a multitrophic parasitoid guild. Aust J Entomol 48:282–286CrossRefGoogle Scholar
  29. Ridenbaugh RD, Barbeau E, Sharanowski BJ (2018) Description of four new species of Eadya (Hymenoptera, Braconidae), parasitoids of the Eucalyptus tortoise beetle (Paropsis charybdis) and other Eucalyptus defoliating leaf beetles. J Hymenoptera Res 64:141–175CrossRefGoogle Scholar
  30. Samuelson GA (1973) Alticinae of oceania (Coleoptera: Chrysomelindae). Pac Insects Monogr 30:1–165Google Scholar
  31. Sands DPA (1997) The ‘safety’ of biological control agents: assessing their impact on beneficial and other non-target hosts. Mem Mus Vic 56(2):611–615CrossRefGoogle Scholar
  32. Smart RK (2017) Reproductive biology and pupal diapause in Eadya sp. (Hymenoptera: Braconidae): a proposed biocontrol agent of Paropsis charybdis Stål (Coleoptera: Chrysomelidae) in New Zealand. Honours, University of Tasmania, HobartGoogle Scholar
  33. Styles JH (1970) Notes on the biology of Paropsis charybdis Stål. (Coleoptera: Chrysomelidae). N Z Entomol 4(3):103–111CrossRefGoogle Scholar
  34. Tanton M, Epila J (1984) Parasitization of larva of Paropsis atomaria Ol. (Coleoptera: Chrysomelidae) in the Australian Capital Territory. Aust J Zool 32:251–259CrossRefGoogle Scholar
  35. Turlings TCJ, Wackers FL, Vet LEM, Lewis WJ, Tumlinson JH (1993) Learning of host-finding cues by hymenopterous parasitoids. In: Papaj DR, Lewis AC (eds) Insect learning: ecology and evolutionary perspectives. Chapman & Hall, New York, pp 51–78CrossRefGoogle Scholar
  36. van Driesche RG, Hoddle MS (2016) Non-target effects of insect biocontrol agents and trends in host specificity since 1985. CAB Rev 11:1–66CrossRefGoogle Scholar
  37. van Lenteren J, Cock MJW, Hoffmeister TS, Sands DPA (2006) Host specificity in arthropod biological control, methods for testing and interpretation of the data. In: Bigler F, Babendreier D, Kuhlmann U (eds) Environmental impact of invertebrates for biological control of arthropods: methods and risk assessment. CABI Publishing, Oxon, pp 38–63CrossRefGoogle Scholar
  38. Withers TM, Barton Browne L (2004) Chapter 5. Behavioral and physiological processes affecting the outcome of host range testing. In: van Driesche RG, Reardon R (eds) Assessing host ranges for parasitoids and predators used for classical biological control: a guide to best practice, forest health technology enterprise team, Report FHTET-2004-03, September 2004. Morgantown, USDA Forest Service, pp 40–55Google Scholar
  39. Withers TM, Allen GR, Reid CAM (2015) Selecting potential non-target species for host range testing of Eadya paropsidis. N Z Plant Prot 68:179–186Google Scholar
  40. Withers TM, Todd JH, Gresham BA, Barratt BIP (2018) Comparing traditional methods of test species selection with the PRONTI tool for host range testing of Eadya daenerys (Braconidae). N Z Plant Prot 71:221–231Google Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2019

Authors and Affiliations

  • T. M. Withers
    • 1
    Email author
  • C. L. Todoroki
    • 1
  • G. R. Allen
    • 2
  • A. R. Pugh
    • 1
  • B. A. Gresham
    • 1
  1. 1.ScionRotoruaNew Zealand
  2. 2.Tasmanian Institute of Agriculture, University of TasmaniaHobartAustralia

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